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The applicant must ensure that the trial seed and/or plant
material are transported in clearly identified, secure containers and are
kept separate from other seed and/or plant material.
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Seeding, transplanting and site maintenance machinery and
equipment must be cleaned at the trial site to prevent dispersal of plant
material. Surplus seed or seedlings, and any plant material remaining
after transplantation, that is to be destroyed, will be disposed of by
autoclaving, burning, or burial at a depth of one metre. Composting of
this material is not an acceptable destruction method.
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In the case of accidental release, recoverable seeds or
seedlings must be collected and destroyed, the site must be marked and
monitored, and the PBO notified immediately. Plants from unrecoverable
seed or seedlings must be mechanically or chemically destroyed.
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B. juncea plants in the trial must be
reproductively isolated from Brassica species by: (a) a minimum 200
metre isolation distance; or (b) growth in cages or bags. Brassica
species include: Brassica napus (Argentine rape, swede rape),
Brassica rapa (oilseed rape, Polish canola, turnip, bird rape),
Brassica juncea (brown mustard, Indian mustard), Brassica carinata
(Ethiopian mustard), Brassica nigra (black mustard), Brassica
hirta [also known as Sinapis alba (white mustard)], Brassica
oleracea (cabbage, cauliflower, brussels sprouts, broccoli, Chinese
cabbage, kale, kohlrabi).
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Trial plants must also be reproductively isolated from
weedy relatives by a minimum of 50 metres, unless other methods of
reproductive isolation has been successfully used. The following species
within a 50 metre radius of the trial site (including ditches,
shelterbelts and neighbouring land) must be removed: Diplotaxis muralis
(sand rocket, stinking wall rocket), Raphanus raphanistrum (wild
radish), Erucastrum gallicum (dog mustard) and Sinapis arvensis
(wild mustard). All listed related weed species must be removed before
seed set when found on the trial site during the year of the trial. These
related weeds must also be removed from the trial site before flowering
when found during the five post-trial growing seasons.
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In the case of reproductive isolation distance for
confined research field trials of B. juncea containing a
transformation event introgressed from a different Brassica species
in which that event is approved for unconfined release, the isolation
distance from the donor species and B. juncea can be reduced. For
example: A novel trait "event X", approved in B. napus is
backcrossed into B. juncea. The new plant line is considered to be
a novel plant and must be grown in confined research field trials until
unconfined release is granted by CFIA. In such cases the reproductive
isolation distance between the confined B. juncea trials and
adjacent B. napus can be reduced from 200 metres to 3 metres. If
B. rapa with the same transformation event has been granted unconfined
release the same principle applies with regard to isolation distance
between the B. juncea confined research field trials and adjacent
B. rapa. Note: This principal does not supercede the Canadian Seed
Growers Association (CSGA) rules for the production of pedigree seed.
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Measurements from permanent surrounding landmarks must be
provided for precise location of the site. Markers must also be placed at
all corners of the trial site to identify the confined field trial
boundaries. The markers must be obvious, identifiable and in place for the
growing seasons of both the trial and the post-harvest restriction period.
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Global Positioning System (GPS) coordinates must be taken
precisely at all corners of each trial site. The GPS coordinates of each
confined research field trial site location must be submitted to the PBO
within 7 days after planting.
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If a chemical treatment is used on the trial site that
requires a time until safe entry, a sign must be posted at the access to
the trial indicating the date and time of spraying as well as the time
until safe entry. This condition is intended to protect the health and
safety of the CFIA inspection staff.
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During the trial growing season the trial site, including
the surrounding isolation distance if applicable, must be monitored at
least weekly to ensure that all related species are removed.
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No plant material from these trials may enter the human
food or livestock feed chain unless approved by Health Canada or the Feeds
Section, CFIA, respectively.
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Harvesting machinery and equipment will be cleaned of all
residual plant material at the trial site prior to being moved to other
locations. Plant material harvested, that is not to be retained, must be
destroyed by burning, autoclaving, or burial to a depth of one metre.
Composting of this material is not an acceptable destruction method.
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Harvested seed and/or propagable plant material from the
confined research field trial may only be retained if requested in the
application and authorized by the PBO. Any harvested seed and/or plant
material must be clearly labelled, securely transported, and stored
separately from other seed and/or plant material.
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Applicants must provide the PBO in writing within 15
working days after harvest with information on:
- quantity of seed and/or plant material harvested at the trial sites
- date(s) of harvest
- quantity of seed and/or plant material disposed of
- location, method and date of disposal
- quantity of seed and/or plant material retained and stored
- storage location and method
Disposal of plant material (propagable and/or non-propagable)
includes harvested plant material as well as residual plant material on
the trial site.
If a trial is destroyed prior to harvest applicants must
provide the PBO in writing within 15 working days after destruction with
information on the trial’s growth stage at the time of destruction, as
well as the date and method of destruction.
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A detailed trial log book must be kept. Records of the
confined research field trial, including current season and post-harvest
site monitoring, activities related to the trial site compliance, cleaning
of machinery, transportation, disposition and storage of all surplus seed
and harvested seed and plant material, must be maintained by the applicant
and made available to the CFIA upon request. A report summarizing the
completed trial and experimental data, including any amendments to the
original protocol, must also be made available to the CFIA upon request.
Detailed records requirements can be found in section 3.8 of Regulatory
Directive 2000-07.
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Seed or other propagable plant material from the confined
research field trial must be harvested unless otherwise approved by the
PBO. Plants must be harvested before full maturity to minimize silique
shattering and seed dispersal. All plant residue remaining on the trial
site must be soil incorporated or destroyed by incineration as soon as
possible after harvest. Applicants are encouraged to destroy all non-propagable
(residual) plant material in a manner whereby the material is not easily
distributed by wind or local fauna yet does not promote seed dormancy. If
the applicant decides to burn the plant material, incineration must be
complete.
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The trial site, including a minimum 10 metre zone (50
metre if a large combine or combination was used during harvest) around
the trial site, must not be seeded to Brassica species for five
years following harvest of the trial. During the five post-trial growing
seasons the trial site, including the 10 metre zone, must be monitored at
least once every two weeks to ensure that all volunteer plants and related
species are removed before flowering.
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Applicants must notify the PBO in writing of crop species
planted on trial sites for each year the sites are subject to post-harvest
restriction. This notification must be received every year by June 15.
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At the applicant’s request, non-modified B. juncea
trials located within 200 metres of the B. juncea confined research
field trials described in Appendix I of the authorization letter are
allowed to proceed to the condition that seed from these non-modified
trials be treated as seed from plants with novel traits . The present
Terms and Conditions 7 to 9 and 11 to 18 apply to these non-modified
trials.