Animal Cell Technology

Dr. Amine Kamen
Tel.: (514) 496-2264
Fax: (514) 496-6785
E-mail: amine.kamen@cnrc-nrc.gc.ca
biography

Group Fact Sheet (pdf)

The Animal Cell Technology group is actively involved in research and development of animal cell bioprocesses for the production of recombinant proteins. The production of such proteins from cloned genes makes it possible to give proteins specialized functions, targeting specific cells or tissues. As a result, the in vitro propagation of animal cells has many applications in the development of biopharmaceuticals and cell and gene therapies. The Animal Cell Technology group initially developed its reputation for excellence in the field of insect cell culture. It has since expanded its activities to include mammalian and human cell processes. The group develops high cell-density processes for the production of recombinant proteins and viral vectors, by growing suspension cultures in serum-free media in different conditions. The group's approaches include development of a Proprietary Human Cell line (HEK293) adapted to suspension and serum free medium which is grown at high cell densities in proprietary medium. This cell line has been tested in batch, fed-batch and perfusion at bioreactor scales up to 100 L. In addition, the research team has developed mammalian cell perfusion processes using transformed mammalian cells and hybridoma. Insect cell cultures have been developed for the production of recombinant proteins and viral vectors using the baculovirus expression system or stable cell lines.

Benslimane C, Elias CB, Hawari J, Kamen A (2005) Insights into the Central Metabolism of Spodoptera frugiperda (Sf-9) and Trichoplusia ni (High-Five™) Insect Cells by Radiolabelling Studies. Biotechnol. Prog. 21: 78-86.

Kamen A, Henry O (2004) Development and Optimization of an Adenovirus Production Process. J. Gene Med. 6: S184-S192.

Durocher Y, Perret S, Kamen A (2002) High-level and High-throughput Recombinant Protein Production by Transient Transfection of Suspension-growing Human 293-EBNA1 Cells. Nucleic Acids Res. 30: 2E9.

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