Microbial & Enzymatic Technology

Dr. Denis Groleau
Tel.: (514) 496-6186
Fax: (514) 496-7251
E-mail: denis.groleau@cnrc-nrc.gc.ca
biography

Group Fact Sheet (pdf)

The Microbial and Enzymatic Technology group identifies, optimizes and scales-up bioprocesses using either microorganisms or enzymes. The group uses an integrated approach, going from DNA to pilot scale fermentation (up to 1500 L) followed by primary recovery and protein/product purification. The microorganisms used are either wild types or mutated and/or recombinant. Projects are focused on methylotrophic microorganisms (bacteria and yeasts), although processes with other microorganisms are also developed. With respect to enzymes, emphasis is on synthetic reactions using various hydrolases. In addition to R&D;, the group has numerous interactions with both industry and the scientific community, at the national and international levels. Processes have been developed for the production, purification and characterization of various products such as: biopharmaceuticals, enzymes, biopolymers, secondary metabolites and green products (bioproducts). The group offers a one-stop approach to product/process development, from molecular biology to bioprocess scale-up and optimization. A portion of the group's effort is also devoted to training highly qualified personnel in modern bioprocessing.

Trani M, Ducret A, Lortie R (2004) Influence of Water-miscible Solvents on Hydrolytic Activity of Crude Almond ß-glucosidase. J. Mol. Catal. B Enzym. 28: 15-18.

Bélanger L, Figueira MM, Bourque D, Morel L, Béland M, Laramée L, Groleau D, Miguez CB (2004) Production of Heterologous Protein by Methylobacterium extorquens in High Cell Density Fermentation. FEMS Microbiol. Lett. 231: 197-204.

Hagedorn A, Levadoux W, Groleau D, Tartakovsky B (2004) Evaluation of Multiwavelength Culture Fluorescence for Monitoring the Aroma Compound 4-hydroxy-2(or 5)-ethyl-5(or 2)-methyl-3(2H)-furanone (HEMF) Production. Biotechnol. Prog. 20: 361-367.

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