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Plants > Plant Protection Policy Directives > Potatoes
File SUBJECTThis directive describes the requirements for the production, maintenance, multiplication and certification of Nuclear Stock class seed potatoes under Canada's national seed potato certification program. It sets out the technical standards for the initiation, maintenance and multiplication of in-vitro plantlets, micro-tubers and mini-tubers, including standards for pathogen testing, varietal purity, physical facilities and documentation. This revision was made to clarify a number of procedural changes related to new activities and developments in the seed potato certification program. Those activities and developments include:
Table of ContentsReview Introduction 1.0 General Requirements 2.0 Policy 3.0 Appendices |
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This directive will be reviewed every three years unless otherwise needed. The next review date for this directive is November 23, 2007. The contact for this directive is Joanne Rousson. For further information or clarification, please contact the Potato Section.
Approved by:
| _______________________________ Director Plant Health Division |
Amendments to this directive will be dated and distributed as outlined in the distribution below.
Canada’s seed potato certification program is based on the continuous input of Nuclear Stock class seed potatoes, i.e., potatoes that have been produced from pathogen-tested tissue culture plantlets under protected environment conditions. Nuclear Stock class seed potatoes, when planted in the field for the first time, will produce Pre-Elite class seed, provided the crop remains visibly free of disease symptoms and varietal purity and meets the requirements of the Seeds Regulations Part II, as determined by the appropriate number of field inspections and laboratory analysis.
In subsequent years of multiplication, the certification class of the progeny seed is lowered to Elite I, Elite 2, Elite 3, Elite 4, Foundation and Certified class, with each class having to meet standards specified by the Seeds Regulations Part II for varietal purity and disease tolerances. All seed classes, except Certified, may be used for further seed production. Certified class seed can only be used to produce ware potatoes. The regulated and mandatory application of these principles for seed potato production provides for a “limited generation” or “flush-through” certification system.
All seed potatoes produced in Canada are inspected in accordance with national inspection standards and, when required, representative samples of lots are analysed in Canadian Food Inspection Agency (CFIA)-accredited laboratories, in order to determine compliance with requirements and tolerances pursuant to the Seeds Regulations Part II.
| Scope | This directive is intended for use by Canadian Food Inspection Agency staff and Canadian producers of Nuclear Stock class seed potatoes. |
| References | Department of the Secretary of State of Canada. The Canadian Style: A Guide to
Writing and Editing. Toronto, 1993. This directive supercedes D-97-08 (Original). |
| Aseptic Environment | An environment which excludes microorganisms that are capable of causing
contamination or infection |
| Aseptic Facility | A facility capable of producing potatoes in an aseptic environment. |
| Breeder's Selection seed potatoes |
Seed potatoes that are direct progeny of true seed, or of selected tubers,
and that are grown for the purpose of evaluation as a potential variety for commercial
use. |
| CFIA | Canadian Food Inspection Agency, established by section 3 of the Canadian
Food Inspection Agency Act |
| Clone | A genetically identical group of plants derived and maintained from one
individual by vegetative propagation. |
| Crop | Breeders Selection seed potatoes, or Breeder's Selection seed potatoes, or
a variety and class of seed potatoes, growing in an aseptic environment, a protected
environment or in one or more fields of a farm unit. |
| Farm Unit | (a) A single tract of land operated for the production and marketing of
seed potatoes under the control of a grower, or (b) A number of separate tracts of land operated as a single unit, with the use of common equipment, facilities or storage, for the (b) A number of separate tracts of land operated as a single unit, with the use of common equipment, facilities or storage, for the production and marketing of seed potatoes under the control of the same grower |
| Lot | The quantity of harvested seed potatoes of a variety and class that is
identifiable by one certificate number or the quantity of BreedersSelection seed potatoes
that are identifiable by one certificate number or the quantity of Breeder's Selection
seed potatoes that is identifiable by one certificate number. |
| Plantlet | A small potato cutting or rooted potato cutting produced under aseptic
conditions using in-vitro propagative techniques |
| Protected Environment | A facility for which there are appropriate procedures and physical barriers
to prevent the entry of plant pathogens and insects. |
| Micro-tuber | A small potato tuber produced under aseptic conditions using in-vitro
propagative techniques. |
| Mini-tuber | A small potato tuber produced in a soil-free medium within a protected environment. |
Seeds Act, (R.S. 1985, c. S-8)
Seeds Regulations (C.R.C., c. 1400), Sections 45-62
Canadian Food Inspection Agency Fees Notice, Canada Gazette, Part 1 (05/13/2000)
The CFIA is charging fees in accordance with the Canadian Food Inspection Agency Fees Notice where applicable. Further information regarding fees can be obtained from a local CFIA office or from the CFIA website.
Three phases are recognised in the production of Nuclear Stock class seed potatoes:
Initiation: where pathogen-tested potato propagules are established in an aseptic
facility through in-vitro micropropagation (i.e., tissue culture)
Maintenance: where pathogen-tested potato propagules are maintained in an aseptic facility
Multiplication: where pathogen-tested potato propagules are multiplied in an aseptic
facility and/or in a protected environment (e.g., greenhouse, screenhouse, growth chamber,
etc.)
Any potato material (i.e., any potato plant part, including stems and tubers) is acceptable for the initiation of and certification as Nuclear Stock class seed potatoes, providing the following requirements have been met.
2.1.1 An application for seed potato crop inspection (CFIA 1317) must be submitted to
the local CFIA office, pursuant to the Seeds Regulations Part II. If the farm
unit also produces Nuclear Stock class seed potatoes in a protected environment, and/or
field grown seed potatoes of other classes, it is recommended that the lots being
initiated or otherwise produced in the aseptic facility be listed on a separate page of
the submitted application, clearly marked to identify the method of production being
utilised.
Note: A grower submitting separate applications for field-grown crops and
crops produced in a protected and/or aseptic environment with the intention of operating
them as separate farm units (i.e., with different grower numbers) is expected to pay the
applicable fees for each application.
2.1.2 The initiation facility must be inspected by a CFIA inspector at least once during active production. All areas involved in the propagation (initiation) of the Nuclear Stock class seed potatoes must be inspected, including: laminar flow cabinets, growth rooms, media preparation areas, etc. This inspection should be recorded using the Nuclear Stock aseptic facility inspection report (CFIA 5292; Appendix 2). If the facility does not meet program requirements, a notification of Nuclear Stock production non-compliance and requirement for corrective action (CFIA 5294; Appendix 4) should be completed and a copy provided to the grower as soon as possible.
2.1.3 The material being initiated must be of a known varietal/clonal identity and must
be duly documented with respect to its origin (name and address), date received, plant
breeders’ rights, plant variety protection, patent or other known aspects of clonal
protection.
Note: provision of documentation in support of the identity of a
particular variety or clone is the responsibility of the initiating facility, not the CFIA.
2.1.4 A sample of at least two plantlets per variety/clone being initiated must be
tested in a laboratory accredited by the CFIA and found not to be infected with any of the
following organisms:
___________________________
1Since the requirement for PMTV testing is new for nuclear
stock multiplication in an aseptic facility, a grace period of a year has been granted.
Therefore starting November 1st, 2005 PMTV testing results will be required to support
certification.
2.1.5 The initiating facility must use recognised aseptic initiation and propagation procedures (i.e., follows procedures and uses equipment which will maintain sterile conditions). Each facility must have a propagation procedure manual for this purpose, for reference by staff as necessary.
2.1.6 If the Centre of Expertise for Potato Diseases confirms the presence of C. m. sepedonicus, in aseptic culture production, all aseptic culture progeny production of the affected variety/clone must be removed and destroyed from the facility. All other aseptic clonal production for the affected facility will be subject to re-testing to verify freedom from C. m. sepedonicus. The level of testing will be a minimum of 2 plantlets per variety/clone.
2.1.7 If the Centre of Expertise for Potato Diseases confirms the presence of PSTVd, or if it is otherwise determined by a CFIA inspector that any of the variety/clone are infected with PSTVd, the infected variety/clone will not be eligible for certification. Any variety/clone that tests positive for PSTVd must be removed from the facility immediately and be destroyed.
2.1.8 If the Centre of Expertise for Potato Diseases confirms the presence of C. m sepedonicus, in other field grown crops under the same farm unit, all field grown crops will have certification revoked. However, an inspector for CFIA may determine, based on verification of aseptic techniques and the absence of contact with field grown crops, that this disease did not affect the Nuclear Stock production and it retains its certification status.
2.1.9 Any variety/clone that tests positive for any other regulated pathogen (other than the one listed in section 2.1.4), any propagative material showing symptoms or signs of any non-regulated endophytic, epiphytic or saprophytic bacterial or fungal infection or other presence of such organisms, and any containers used in the propagative process showing signs of bacterial or fungal contamination, must be removed from the facility immediately and be sterilized or destroyed.
2.1.10 The initiating facility must maintain an information system which accurately
documents the following information for each variety/clone in the facility:
The following requirements must be met for the maintenance of Nuclear Stock propagative material in an aseptic facility.
2.2.1 The facility must use recognised aseptic propagation procedures; (i.e., must follow procedures and use equipment which will maintain sterile conditions).
2.2.2 Varietal or clonal integrity must be maintained by following procedures which will prevent varietal mixture during all transfer, storage and handling activities.
2.2.3 There are no requirements for specific disease testing during the maintenance phase other than those requirements described in section 2.2.5.
2.2.4 Maintenance within the initiating facility requires physical and clearly identifiable separation to be present between the material certified as Nuclear Stock class seed potatoes and all other material.
2.2.5 Where maintenance takes place in a facility other than the initiating facility, the material must be certified as Nuclear Stock class seed potatoes prior to maintenance (i.e., must comply with all components of section 2.1 or 2.3 and possess disease testing results as described in section 2.1.4 or 2.3.4).
2.2.6 If the Centre of Expertise for Potato Diseases confirms the presence of C. m. sepedonicus, in aseptic culture production, all aseptic culture progeny production of the affected variety/clone must be removed and destroyed from the facility. All other aseptic clonal production for the affected facility will be subject to re-testing to verify freedom from C. m. sepedonicus. The level of testing will be a minimum of 2 plantlets per variety/clone.
2.2.7 If the Centre of Expertise for Potato Diseases confirms the presence of C. m sepedonicus, in other field grown crops under the same farm unit, all field grown crops will have certification revoked. However, an inspector for CFIA may determine, based on verification of aseptic techniques and the absence of contact with field grown crops, that this disease did not affect the Nuclear Stock production and it retains its certification status.
2.2.8 If the Centre of Expertise for Potato Diseases confirms the presence of PSTVd, or if it is otherwise determined by a CFIA inspector that any of the variety/clone are infected with PSTVd, the infected variety/clone will not be eligible for subsequent certification as Nuclear Stock class seed potatoes. Any variety/clone that tests positive for PSTVd must be removed from the facility immediately and be destroyed.
2.2.9 Any variety/clone that tests positive for any other regulated pathogen (other than the one listed in section 2.1.4), any propagative material showing symptoms or signs of any non-regulated endophytic, epiphytic or saprophytic bacterial or fungal infection or other presence of such organisms, and any containers used in the propagative process showing signs of bacterial or fungal contamination, must be removed from the facility immediately and be sterilized or destroyed.
2.2.10 The facility must maintain an information system which accurately documents the following information for each variety/clone being maintained:
2.3 Multiplication in an Aseptic Facility
The following requirements must be met for the multiplication of Nuclear Stock class seed potatoes in an aseptic facility to be approved and certified by the CFIA.
2.3.1 An application for seed potato crop inspection (CFIA 1317) must be submitted to the local CFIA office, pursuant to the Seeds Regulations Part II. If the farm unit also produces Nuclear Stock class seed potatoes in a protected environment, and/or field grown seed potatoes of other classes, it is recommended that all lots being produced in the aseptic facility be listed on a separate page of the submitted application, clearly marked to identify the method of production being utilised.
Note: A grower submitting separate applications for field-grown crops and crops produced in a protected and/or aseptic environment with the intention of operating them as separate farm units (i.e., with different grower numbers) is expected to pay the applicable fees for each application.
2.3.2 The multiplication facility must be inspected by a CFIA inspector at least once during the multiplication phase. All areas involved in the propagation of the Nuclear Stock class seed potatoes must be inspected, including: laminar flow cabinets, growth rooms, media preparation areas, etc. This inspection should be recorded using the Nuclear Stock aseptic facility inspection report (CFIA 5292; Appendix 2). If the facility does not meet program requirements, a notification of Nuclear Stock production non-compliance and requirement for corrective action (CFIA 5294; Appendix 4) should be completed and a copy provided to the grower as soon as possible.
2.3.3 The multiplication facility must use recognised aseptic propagation procedures; (i.e., must follow procedures and use equipment which will maintain sterile conditions). Each facility must have a propagation procedure manual for this purpose, for reference by staff as necessary.
2.3.4 Except for micro-tuber production (where valid disease testing results are required prior to the initiation of the micro-tuber production cycle), each crop under production must have valid disease testing results at all times during the multiplication process. Tests must be carried out on a minimum of two plantlets for each variety/clone. They must be carried out by a laboratory accredited by the CFIA and are valid for a period of twelve months. For clarification in determining this period, the recognised twelve month period will commence on the date that the sample was submitted for testing to the accredited laboratory. This date should be included on the official laboratory testing report. Each crop must have been found not to be infected with any of the following organisms:
2.3.5 If the Centre of Expertise for Potato Diseases confirms the presence of C. m. sepedonicus, in aseptic culture production, all aseptic culture progeny production of the affected variety/clone must be removed and destroyed from the facility. All other aseptic clonal production for the affected facility will be subject to re-testing to verify freedom from C. m. sepedonicus. The level of testing will be 1% or a minimum of 5 plantlets to a maximum of 50 plantlets per clone.
2.3.6 If the Centre of Expertise for Potato Diseases confirms the presence of C. m sepedonicus, in other field grown seed crops under the same farm unit, all field grown crops will have certification revoked. However, an inspector for CFIA may determine, based on verification of aseptic techniques and the absence of contact with field grown crops, that this disease did not affect the Nuclear production and it retains its certification status.
2.3.7 If the Centre of Expertise for Potato Diseases confirms the presence of PSTVd, or if it is otherwise determined by a CFIA inspector that any of the variety/clone are infected with PSTVd, the infected variety/clone will not be eligible for certification. Any variety/clone that tests positive for PSTVd must be removed from the facility immediately and be destroyed.
2.3.8 Any variety/clone that tests positive for any other regulated pathogen (other than the one listed in section 2.3.4), any propagative material showing symptoms or signs of any non-regulated endophytic, epiphytic or saprophytic bacterial or fungal infection or other presence of such organisms, and any containers used in the propagative process showing signs of bacterial or fungal contamination, must be removed from the facility immediately and be destroyed or sterilized.
2.3.9 The multiplication facility must maintain an information system which accurately documents the following information for each variety/clone being multiplied:
2.4 Multiplication in a Protected Environment
The following requirements must be met for the multiplication of Nuclear Stock class seed potatoes in a protected environment to be approved and certified by the CFIA.
2.4.1 An application for seed potato crop inspection (CFIA 1317) must be submitted to the local CFIA office, pursuant to the Seeds Regulations Part II. If the farm unit also initiates or produces Nuclear Stock material in an aseptic facility, and/or produces field grown seed potatoes of other classes, it is recommended that all lots being produced in the protected environment be listed on a separate page of the submitted application, clearly marked to identify the method of production being utilised.
Note: A grower submitting separate applications for field-grown crops and crops produced in a protected and/or aseptic environment with the intention of operating them as separate farm units (i.e., with different grower numbers) is expected to pay the applicable fees for each application.
2.4.2 The grower must notify an inspector of the CFIA prior to the proposed date of planting in order to allow sufficient time for a pre-planting inspection to be scheduled, if deemed necessary (approximately two weeks advance notice may be sufficient, depending on the demands on the local CFIA office). The protected environment may be required to be inspected and approved by a CFIA inspector (this is at the inspector’s discretion) before planting can proceed. This pre-planting inspection, and approval to plant, should be recorded using a Nuclear Stock protected environment inspection report (CFIA; 5293; Appendix 3). If the protected environment does not meet program requirements, a notification of Nuclear Stock production non-compliance and requirement for corrective action (CFIA 5294) should be completed and a copy provided to the grower as soon as possible.
2.4.3 The facility must be inspected by a CFIA inspector at least once during the growing cycle (this requirement is in addition to the optional pre-planting inspection described in section 2.4.2). The inspection must take place at or about budding/flowering time (40 - 70 days after planting). Depending on the condition or appearance of the crop, the inspector may take leaf samples for laboratory testing to be carried out by the Centre of Expertise for Potato Diseases to determine if the crop is free of regulated pathogens (section 2.4.12) or to confirm varietal purity. This inspection should be recorded using a Nuclear Stock protected environment inspection report (CFIA; 5293; Appendix 3). If the protected environment or growing crop does not meet program requirements, a notification of Nuclear Stock production non-compliance and requirement for corrective action (CFIA 5294) should be completed and a copy provided to the grower as soon as possible.
2.4.4 The protected environment must be “aphid-proof” and be equipped as follows:
2.4.5 The facility must be cleaned and disinfected, and must be free from all potato and solanaceous plant debris prior to planting.
2.4.6 No field-produced seed potatoes (including pathogen tested clonal selections), non-seed potatoes, nor any other solanaceous, pelargonium species or other host plants of Ralstonia solanacearum Race 3, Biovar 2 can be grown in the same protected environment facility. In addition, any quarantine pathogen for seed potatoes identified in the air, water, soil or environment associated with a Nuclear Stock production facility will result in decertification of all seed potato crops grown in that production facility.
2.4.7 The producer must adopt a management program that includes effective sanitation practices and effective insect and disease monitoring and prevention procedures (e.g., use of yellow sticky traps for aphid monitoring, adoption of integrated pest management practices, records of any pests or diseases observed and corrective actions taken, etc.).
2.4.8 Nuclear Stock class propagative material must be planted in commercially available soil-free medium which has not been recycled.
2.4.9 If ground beds are used, the underlying soil must be separated by a new or clean, disinfected physical barrier from the growing medium (e.g., separated by landscape cloth). If containers are used, they must be new, or cleaned and disinfected.
2.4.10 The crop must be grown from certified Nuclear Stock class seed potatoes which were produced in an aseptic facility, or from first generation Nuclear Stock class mini-tubers produced in the grower’s own protected environment facility, or from stem cuttings produced in the grower’s own protected environment facility.
2.4.11 Varieties/clones must be separated by physical barriers which will maintain varietal purity.
2.4.12 Each crop under production must have valid disease testing results at the time of planting in a protected environment, unless planting first generation mini-tubers (section 2.4.13). Tests for the following pathogens must have been carried out by a laboratory accredited by the CFIA and are valid for a period of twelve months. For clarification in determining this period, the recognised twelve month period will commence on the date that the sample was submitted for testing to the accredited laboratory. This date should be included on the official laboratory testing report. Samples must have been collected within 30 days prior to the date of testing. Each crop must have been found not to be infected with any of the following organisms:
2.4.13 If first generation mini-tubers are used to produce a second generation, the first generation must have been tested by a laboratory accredited by the CFIA and found to be free from the pathogens listed in section 2.4.12 If testing on the first generation was not carried out, tests for the pathogens listed in section 2.4.12 must be performed by a laboratory accredited by the CFIA on the second generation crop.
2.4.14 When additional pathogen testing is required on a crop producing mini-tubers, samples should be collected at budding/flowering time (approximately 40 -70 days after planting) on a representative sample consisting of 1% of the plants/tubers with a minimum of 5 and a maximum of 50 plants/tubers sampled per lot. All samples taken for required pathogen testing must be collected under the supervision of a CFIA inspector.
2.4.15 The facility must maintain an information system which accurately documents the following information for the crop(s) within each structure:
2.4.16 If the Centre of Expertise for Potato Diseases confirms the presence of C. m. sepedonicus, in a protected environment facility, all production of the affected facility must be destroyed. However, a CFIA inspector may determine, based on the definition of ‘farm unit’ and verification of sanitation techniques utilised in the protected environment and separation with field grown production, that this disease was not in contact with field-grown production and it may retains its certification status.
2.4.17 If the Centre of Expertise for Potato Diseases confirms the presence of C. m sepedonicus, in other field grown potatoes identified under the same application and definition of ‘farm unit’, all field grown seed potato crops will have certification revoked. However, a CFIA inspector may maintain the certification status of the crops grown in the protected environment, based on the definition of ‘farm unit’, verification of sanitation techniques and the absence of contact between the field grown crops and the ones grown in the protected environment.
2.4.18 If the Centre of Expertise for Potato Diseases confirms the presence of PSTVd, or if it is otherwise determined by a CFIA inspector that any of the variety/clone are infected with PSTVd, the infected variety/clone will not be eligible for certification. Any variety/clone that tests positive for PSTVd must be removed from the facility immediately and be destroyed.
2.4.19 In the event that insect disease vectors (i.e., aphids) are detected by a CFIA inspector on or in the growing crop, including evidence of wingless aphids, nymphs, molting skins, dead and/or parasitized aphids, the grower must provide post-harvest test results to the CFIA. One or two extraneous winged aphid(s) or vector(s) found on sticky traps, or in areas not directly in contact with the crop, does not automatically indicate additional testing requirements, but will alert the inspector to be vigilant during his inspection of the crop, to ensure aphid or vector activity is not occurring in the growing crop.
After confirming the evidence of virus vectors in the crop, a representative sample collected under the supervision of an inspector (consisting of 1% of the harvested tubers with a minimum of 5 and a maximum of 50 tubers sampled per lot), for each variety/clone produced in the protected environment, must be post-harvest virus tested and, provided the results are negative for PVS, PVM, PVA, PVY and PLRV, the crop will be assigned Nuclear Stock class. Failure to provide post-harvest test results, if required to do so, will result in assigning Pre-Elite class to all varieties/clones in the protected environment (assuming all other standards of the class have been met).
2.4.20 If testing performed by a laboratory accredited by the CFIA reveals the presence of PVA, PVS, PVM, PVY, PVX, or PLRV, the crop will be assigned the Pre-Elite class (providing it meets the appropriate standards), not Nuclear Stock class, and allowed to be grown for the next generation by the owner only.
2.5.1 An application for seed potato crop inspection (CFIA 1317) must be filed for each variety/clone produced, as follows:
2.5.1.1 For Nuclear Stock produced in an aseptic facility, the application for certification must be filed before the material is transferred to a new owner or transferred into a protected environment or planted in a field.
2.5.1.2 For Nuclear Stock produced in a protected environment, the application must be filed within thirty days after planting the crop(s) in the protected environment. (NOTE: the requirement to notify the local CFIA office in advance of planting stated in section 2.4.2 to allow for a pre-planting facility inspection, if deemed necessary, still applies). Amendments to the application can be made no later than the appropriate inspection time for the crop growing in the protected environment facility. A subsequent inspection may be required for those crops not at 40-70 days maturity. A new application must be filed for crops not planted at the time of inspection (40-70 days).
For facilities under continuous propagation and production, an arrangement with the local inspection office must be determined and formal notification with that office listing new crops growing in the appropriate window for inspection, must be filed, inspected and recorded on the application for seed potato crop inspection (CFIA 1317) and entered into the CFIA Multi Commodity Activity program database (MCAP). Under these provisions one annual application will suffice.
2.5.2 The application for certification must be made using the application for seed potato crop inspection (CFIA 1317). It is recommended that a separate page of the submitted application be used to differentiate between each of the three following types of production: Nuclear Stock initiation/production in an aseptic facility; Nuclear Stock production in a protected environment; and any field potato production. The completed application package must contain the following information and supporting documentation.
2.5.3 Certification of each variety/clone will be granted by the CFIA, following official inspections, if all the requirements under section 2.1, 2.3 or 2.4 have been met, and the crop meets all the standards of the Seeds Regulations Part II.
2.5.4 Growing Crop Certificates (CFIA 1318) must be issued for all certified potatoes, including Nuclear Stock class seed potatoes, pursuant to the Seeds Regulations Part II.
2.6.1 Nuclear Stock class seed potatoes being transferred to a new owner must be accompanied by one of the following certificates supplied by the CFIA prior to the transfer of the stock:
2.6.2 An application for issuance of either Nuclear Stock Tags or certificates should be made by the producer using an Application for issuance of Nuclear Stock tags and certificates (CFIA 5295; Appendix 5).
2.6.3 The following information must be provided on the Nuclear Stock Tag (CFIA 5298):
2.6.4 The following information must be provided on the Nuclear Stock Certificate (CFIA 4351) or Certificate of Authorisation (CFIA 4378):
2.6.5 Nuclear Stock Tags and Nuclear Stock Certificates can only be used for varieties registered and sold in Canada pursuant to the Seeds Act and Seeds Regulations Part III. Unregistered varieties must not be listed on a Nuclear Stock Tag or Nuclear Stock Certificate, unless they are being exported directly to another country. Transfer of unregistered varieties should be made using a Certificate of Authorisation (CFIA 4378). If unregistered Nuclear Stock varieties are transferred using a Certificate of Authorisation, the information requirements described in section 2.6.4 must also be present.
2.6.6 Nuclear Stock class seed potatoes produced in a protected environment and transferred to a new owner cannot be used to further produce Nuclear Stock class seed potatoes.
Appendix 1: Summary Table
Appendix 2: Nuclear Stock aseptic facility inspection report (CFIA 5292)
Appendix 3: Nuclear Stock protected environment inspection report (CFIA 5293)
Appendix 4: Notification of Nuclear Stock production non-compliance and requirement for
corrective action (CFIA 5294)
Appendix 5: Application for issuance of Nuclear Stock tags and certificates (CFIA 5295).
Summary Table
| CRITERIA | INITIATION (aseptic) |
MAINTENANCE (aseptic) |
MULTIPLICATION (in an aseptic facility) |
MULTIPLICATION (in a protected environment) |
COMMENTS |
| AN APPLICATION FOR CERTIFICATION MUST BE COMPLETED | Must be filed before the material is transferred to a new owner or transferred into a protected environment or planted in a field. | N/A | Must be filed before the material is transferred to a new owner or transferred into a protected environment or planted in a field. | Must be filed within 30 days after the crop(s) have been planted.
See details in section 2.5.1.2 |
Use the application for seed potato crop inspection (CFIA 1317). It is recommended that a separate page be used to differentiate between each type of production. Section 2.5.2 details all the info that the application package must contains. |
| INSPECTION NEED | - At least once during active production. - Recorded on CFIA 5292 |
N/A | - At least once during the multiplication phase. - Recorded on CFIA 5292 |
- At least once, at or about budding / flowering time
(40-70 days after planting) Plus, an optional pre-planting inspection (see 2.4.2) - Recorded on CFIA 5293 |
If the facility does not meet program requirement, use form CFIA 5294 for corrective action. |
| VARIETAL/CLONAL IDENTITY documented | x | x | x | x | Origin must be duly documented. |
| RECOGNISED ASEPTIC INITIATION AND/OR PROPAGATION PROCEDURES | Each facility must have a propagation procedure manual (2.1.5). | The facility must use recognised aseptic propagation procedures. | Each facility must have a propagation procedure manual (2.3.3). | The producer must adopt a management program that includes effective sanitation practices (2.4.7). The protected environment must be "Aphid proof" (2.4.4). | |
| LAB TESTING REQUIREMENTS: FREE OF VIRUS / VIROID / BACTERIA | Minimum of 2 plantlets per variety. Viruses: PVA, PVS, PVM,
PVY, PVX, PLRV, (PotLV, PMTV starting November 1st, 2005) |
No requirements. Except: Where maintenance takes place
in a facility other than the Initiating facility, the material must be certified as
Nuclear Stock class seed potatoes prior to maintenance. (2.2.5) |
Minimum of 2 plantlets per variety (valid for one year, must have valid*
testing results at all time during multiplication process) Viruses: PVA,PVS, PVM, PVY, PVX, PLRV Viroid: PSTVd Bacteria: CMS Except for mico-tuber production where testing results are required prior to initiation of the production cycle. |
Must have valid* testing results at the time of planting
for the following: Viruses: PVA, PVS, PVM, PVY, PVX, PLRV Viroid: PSTVd Bacteria: CMS Except for planting first generation mini-tubers (details in section 2.4.13 and 2.4.14) |
* For clarification on determining the validity period, the recognised twelve month period will commence on the date that the samples was submitted for testing to the lab (must be in a CFIA accredited laboratory). PMTV and PLRV testing are usually done at the Initiation phase and will be required starting November 1st, 2005. |
| IF BRR IS DETECTED | - Destroy all progeny of that clonal line - Retest all other aseptic clonal production in the affected facility. Level of testing: a minimum of 2 plantlets per clone/variety. |
- Destroy all progeny of that clonal line - Retest all other aseptic clonal production in the affected facility. Level of testing: a minimum of 2 plantlets per clone/variety. |
Destroy all progeny of that clonal line - Retest all other aseptic clonal production in the affected facility. Level of testing: 1% or a minimum of 5 plantlets to maximum of 50 plantlets per clone/variety. |
Destroy all production in the protected environment and conduct an investigation to determine risk of spread to farm unit. | If BRR is detected in field crops, a CFIA inspector may be able to verify aseptic techniques within nuclear production and the absence of contact with field grown crops, to prevent de-certification. |
| IF PSTVd IS DETECTED | - Destroy material from the positive clone/variety | - Destroy material from the positive clone/variety | - Destroy material from the positive clone/variety | - Destroy material from the positive clone/variety | |
| IF positive FOR ANY REGULATED AND NON REGULATED PATHOGEN | - Destroy all positive material | - Destroy all positive material | - Destroy all positive material | - Determine class eligibility for disease tolerance as in Regulations.
(2.4.20) - If evidence of insect disease vector (see section 2.4.19) |
|
| THE FACILITY MUST MAINTAIN AN INFORMATION SYSTEM | (see 2.1.10 for details) | (see 2.2.10 for details) | (see 2.3.9 for details) | (see 2.4.15 for details) | |
| CFIA ISSUED GROWING CROP CERTIFICATE | x | N/A | x | x | Under application and prior to distribution of Nuclear class seed |
| DOCUMENT USED FOR TRANSFER OF MATERIAL | Nuclear Stock Certificates or Certificate of Authorisation (for unregistered varieties) |
N/A | Nuclear Stock Certificates or Certificate of Authorisation (for unregistered varieties) |
Nuclear Stock Certificates or Nuclear Stock Tags or Certificate of Authorisation (for unregistered varieties) |
See section 2.6.3 and 2.6.4 for information to be provided on document
issued. And section 2.6.5 for details about transfer of unregistered varieties. *Nuclear stock class seed potatoes produced in a protected environment and transferred to a new owner cannot be used to further produce Nuclear stock class seed potatoes. |
Nuclear Stock aseptic facility inspection report (CFIA 5292)
Nuclear Stock protected environment inspection report (CFIA 5293)
Application for issuance of nuclear stock tags and certificates (CFIA 5295)
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Important Notices |
