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This module describes background and procedures required to
qualify a donor or teaser animal for entry into an artificial insemination (AI) centre. |
Background Information
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1. |
The animal semen production centre is an establishment in
which animal semen is collected and/or processed under the terms of a permit issued by the
CFIA. |
| Legislation |
2. |
The enabling legislation to draft regulations pertaining to the artificial
insemination industry is contained in Section 64(q) of the Health of Animals Act.
The regulations pertaining to the artificial insemination industry are contained in
Sections 2, 60, 115, 116, 117, 118, 119, 160, and 161 of the Health of Animals
Regulations. |
Procedures to Qualify a Semen Donor or a Teaser Animal for
Entry into an AI Centre
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3. |
All pre-entry qualifying procedures performed on the farm of origin are to
be performed by an accredited veterinarian who has been authorized to conduct those
specific procedures under the authority of an Accredited Veterinarian Agreement. The
veterinarian should contact the respective district veterinarian for advice as to the
submission of samples, the respective laboratory to be used, and the completion of
laboratory forms. Blood samples must be sent to CFIA laboratories, unless otherwise
specified. |
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4. |
Donor animals consigned to a recognized bull sale or exhibition may be
tested on the farm of origin before the event. Following the sale or exhibition, the
animal may enter the designated isolation facility of an approved semen production centre
to commence health testing for entry into the resident herd of the centre. The centre
assumes the risk associated with this procedure. |
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5. |
Note that the period of test validity is 60 days from the
date of sample collection, except for boars destined to an insemination centre approved
for export of semen to the European Community (EC) where the period is only 30
days. In the case of tuberculosis, the date of test is the date on which the
injection site is read. |
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6. |
The accredited veterinarian can apply the procedures in an isolation
facility at a semen production centre if the centre is approved, and if the accredited
veterinarian is authorized to perform on-farm testing for the same species for the
following animals:
- swine arriving in a production centre in which semen is for distribution in Canada,
- cervids,
- bison, and
- sheep and goats when semen is collected in a sire reference program.
In all other cases, qualifying and testing procedures associated with the assessment of
the isolation, health status of semen donor and teaser animals subsequent to their
presentation to the pre-entry isolation facilities of the semen production centre are to
be performed by CFIA staff. |
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7. |
The herd of origin must be inspected and found free from clinical evidence
of infectious disease and, in so far as can be determined, from history of infectious
diseases during the preceding 60 days. |
| Isolation and Identification |
8. |
The donor animals or teasers should remain in isolation at the premises of
origin and must not be exposed to any infection to which they are susceptible from the
date of pre-entry health testing until entry into the designated isolation facility of the
centre. |
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9. |
Animals must be positively identified (See 2.1
Identification of Livestock). |
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10. |
All donor animals being presented as prospective additions to semen
production centres must be examined and any evidence of heritable physical defects is to
be recorded on the health certificate CFIA/ACIA 1634 Certificate of Health for Entry
into a Semen Production Centre. |
| Certification |
11. |
The CFIA/ACIA 1634 certificate must be completed by the accredited
veterinarian who performs the on-farm pre-entry inspection of the animal. It is the
responsibility of the accredited veterinarian to ensure the completed certificate is
endorsed and stamped by the responsible district veterinarian before being issued to the
owner. The distribution of the CFIA/ACIA 1634 is as follows:
- one copy of the certificate is retained by the district veterinarian;
- one copy is sent to the semen production centre for their records; and
- one copy is provided to the owner to accompany the animal to the semen production centre
for entry into the isolation area at the Centre.
Note: Multiple entries of donor boars from the same herd may be listed
on a single annex to the CFIA/ACIA 1634. |
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12. |
Anyone collecting or processing bovine semen-whether operating from a
mobile laboratory or a fixed centre-must be in possession of a "Permit to Operate a
Semen Production Centre" issued by the National Coordinator, Artificial Insemination,
Animal Health and Production Division, Ottawa. A mobile laboratory must be associated with
a permanent facility in which the records referred to in Section 119(2) of the Health
of Animals Regulations are maintained. |
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13. |
Section 115 of the Health of Animals Regulations requires that
every person who owns a premises on which semen is collected and labelled "Owner Use
Only" must be in possession of a "Permit to Collect Semen" issued by the CFIA
area or district office upon application by the owner, unless the owner of the premises is
granted a "Permit to Operate a Semen Production Centre. |
|
References A copy of CFIA/ACIA
1634 is available from your district veterinarian. |
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This module describes requirements for export eligible bovine
semen that is for domestic use and for owner's use only. |
Export Eligible Semen
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Requirements for bovines entering a centre for the
production of semen for export: |
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1. |
The herd of origin must not be subject to any quarantine and the animals
must be subject to the following tests with negative results, unless otherwise specified,
within a 60-day period before entry into the isolation facility of the centre i. Tuberculosis - intradermal purified protein derivative
(PPD) bovine tuberculin - caudal fold read at 702 hours. Any reaction must be reported to
the CFIA veterinarian.
ii. Brucellosis - (serum) tube agglutination test (TAT)
less than 30 IU, and - (serum) complement fixation (CF), negative at 1/5.
iii. Leptospirosis - (serum) microscopic agglutination
test (MAT) - serotypes L. pomona, L. sejroe, L. hardjo, L.
canicola, L. grippotyphosa
and L. icterohaemorrhagiae. With exception of L.
hardjo, for which a negative test at 1:100 is required,
bulls with a test result of 1:100 or 1:200 must be retested after a minimum interval of 14
days and a stable titre obtained to be considered negative and eligible for entry into the
isolation facility of the centre. The sampling dates and titres must be recorded on the
health certificate accompanying the animal to the AI centre.
iv. Bluetongue - (serum) enzyme-linked immunosorbent assay
(ELISA)
v. Johne's disease - (serum) ELISA
vi. Enzootic bovine leucosis (EBL) - (serum) ELISA
vii. Enzootic haemorrhagic disease - Type 2 (EHD2) -
(serum) AGID
viii. Bovine viral diarrhea (BVD) - (serum)
IMMUNOPEROXIDASE. Animals younger than six months of age must be retested by
immunoperoxidase and serum neutralization (SN) after reaching that age. The SN test is to
determine the serological status of the prospective donor bull and seropositive animals
are allowed to enter into the isolation facility of the centre. |
| Optional Test |
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ix. Infectious bovine rhinotracheitis (IBR)
- (serum) ELISA This test is done at the request of the semen production centre; a
negative test is required for animals entering into an IBR negative facility. |
| EBL testing the dam |
2. |
In addition, when possible, the uterine dam of the prospective donor bull
should be subjected to an ELISA test for EBL subsequent to the weaning of the prospective
donor, with negative results. This test of the uterine dam is required to export semen
to the EC Member States when semen is collected from a donor bull before he reaches 24
months of age and obtains a negative result to an ELISA test after reaching that age. This
test is not required when the prospective donor bull originates from a Canada Health
Accredited Herd for EBL (CHAH-EBL). |
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3. |
A CFIA/ACIA 1634 certificate must accompany the animal to the
isolation facility of the centre (Refer to 10.1 Artificial
Insemination - General Information paragraph 11 for distribution). |
Domestic Use Semen
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Requirements for bovines entering a semen
collection centre for the purpose of the production of semen for distribution in Canada: |
| Herd of Origin |
4. |
The herd of origin must not be subject to any quarantine and the animals
must be subject to the following tests with negative results, unless otherwise specified,
within the 60-day period preceding entry into the isolation facility of the centre. |
| Required Tests |
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i. Tuberculosis - intradermal purified
protein derivative (PPD) bovine tuberculin - caudal fold read at 72 hours. Any reaction
must be reported to the CFIA veterinarian.
ii. EBL - (serum) ELISA
iii. Johne's disease - (serum) c-ELISA
iv. BVD - (serum) IMMUNOPEROXIDASE Animals younger than
six months of age must be retested after reaching that age. |
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5. |
A CFIA/ACIA 1634 certificate must accompany the animal to the
isolation facility of the centre (Refer to paragraph 11 in Module 10.1
for distribution). |
Owner's Use Only Semen
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Requirements for bovines collected for owner's use
only: |
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6. |
This applies to semen intended for the use of the owner of the donor animal
in their own herd and may be collected from bulls at a semen collection centre approved
for the purpose, or alternatively, may be collected on the premises where the donor is
kept and processed in an approved mobile laboratory. |
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7. |
This section does not apply to semen being collected for the purpose of
evaluating the fertility of the donor that is not intended to be inseminated into a
female. |
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8. |
Bovine semen collected for any purpose other than for use in the owner's
own herd must be collected in an approved semen collection centre. |
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9. |
All semen collected under the provisions of "Owner's Use Only
Semen" should be clearly labelled "Owner's Use Only" with the
identification of the donor animal, breed, identity of the collection unit, and date of
collection of the semen. The name of the owner or herd of the donor bull is optional.
Semen should be evaluated and processed according to current industry standards. |
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10. |
Semen labelled "Owner's Use Only" is to be stored at the farm of
origin or in approved storage facility dedicated to that purpose. Semen of this status
must never be stored in the same container as semen intended for distribution in Canada or
in containers dedicated to the storage of semen for export. An exception is made for the
semen stored at the farm of origin where semen "Owner's Use Only" is allowed to
be stored in the same container as the semen purchased by the owner for use in his herd. |
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11. |
IMPORTANT: Semen labelled "Owner's Use Only" must not
be traded or sold. It may be used in the herd(s) of the registered owner of the donor
bull only. Health of Animals Regulations require that records of the collection, use,
distribution, and storage must be kept and made available to a CFIA inspector. Issuance of
a permit to collect semen permits the CFIA to conduct an audit of the owner's semen
storage and records of semen use. |
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This module describes requirements for export eligible porcine
semen that is for domestic use and owner's use only. |
Porcine
Export Eligible Semen
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Requirements for boar intended for entry into a
semen collection centre to produce semen for export: |
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1. |
Identification can be either an official HofA tag, a legible tattoo, or a
plastic tag with identification of the farm of origin and a unique number for the animal.
Animals improperly identified or that have lost their identification will be rejected when
they arrive at the isolation facility of the AI centre and retested at the centre's
expense. |
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2. |
The herd of origin must not be subject to any quarantine and the animals
must be subject to the following tests with negative results, unless otherwise specified,
within the 60 days preceding entry into the isolation facility of the centre: Note: Centres approved for export to the EC must have animals
tested within the 30 days preceding entry into the isolation facility of
the centre.
i. Brucellosis - (serum) BPAT and TAT and CF test at 1/5.
Note: Centres approved for export to China must have all
animals obtain a result of less than 30 IU when tested by TAT.
ii. Leptospirosis - (serum) Microagglutination test (MAT)
- L. pomona and L. sejroe.
Boars with a test result of 1:100 or 1:200 must be retested after a minimum interval of 14
days and obtain a stable titre to be considered negative and eligible for entry into the
isolation facility of the centre.
iii. Transmissible Gastroenteritis (TGE) - (serum) serum
neutralisation (SN) test or ELISA test. In the event of a positive reaction to the SN
test, the laboratory conducts an ELISA test; if the ELISA test is negative, the animal is
eligible to enter the isolation facility of the centre.
iv. Aujesky's disease - (serum) ELISA test. |
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3. |
A CFIA/ACIA 1634 Certificate of Health for Entry into a Semen
Production Centre must accompany the animal to the isolation facility of the centre.
(Refer to paragraph 11 of 10.1 Artificial Insemination - Background
Information for distribution.) |
Domestic Use Semen
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Requirements for boar intended for entry into a
semen collection centre to produce semen for distribution in Canada (This semen is not
eligible for export and may only be used within Canada.): |
|
4. |
Identification can be an official HofA ear tag, a legible tattoo, or a
plastic tag with identification of the farm of origin and a unique number of the animal.
Animals improperly identified or that have lost their identification will be rejected upon
arrival at the isolation facility of the centre and will be retested at the expense of the
centre. |
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5. |
The herd of origin must not be subject to any quarantine and the animals
must be subject to the following test with negative results, unless otherwise specified,
within the 60 days before entry into the isolation facility of the centre: i.
Transmissible Gastroenteritis - (serum) SN or ELISA test.
In the event of a positive reaction to the SN test, the laboratory conducts
an ELISA test; if the ELISA test is negative, the animal is eligible to remain in the
centre. |
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6. |
A CFIA/ACIA 1634 Certificate of Health for Entry into a Semen
Production Centre must accompany the animal to the isolation facility of the centre.
(Refer to paragraph 11 of Module 10.1 for distribution
details.) |
Isolation Health Requirements of Semen Donor Boar
(For Distribution in Canada only)
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7. |
All qualifying and testing procedures associated with the assessment of the
isolation health status of semen donor boars may be performed by a veterinarian authorized
to perform these functions as an accredited veterinarian. |
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8. |
All animals presented for entry as additions to the resident herd of a
semen production centre must undergo a minimum period of 30 days in an isolation facility
maintained by the centre; the isolation period may begin when all animals are present in
the isolation facility. |
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9. |
An animal is to be considered eligible for entry into isolation only if a
certificate of health completed by an accredited veterinarian as per paragraph 6 above
accompanies the animal to the centre. |
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10. |
An identification check is conducted on each animal against the certificate
of health presented by the centre. Animals improperly identified or that have lost their
identification will be rejected upon arrival at the isolation facility of the centre and
retested at the expense of the centre. |
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11. |
All animals presented for entry as additions to the semen production centre
must receive an injection of dihydrostreptomycin (25 mg per kg of body weight) or an
alternate antibiotic at the dosage recommended by the manufacturer, as a preventive
measure against leptospirosis. |
|
12. |
While in the isolation facility and after a minimum of 30 days in
isolation, the pre-entry test must be repeated with negative results, unless otherwise
specified, as follows: i. TGE - (serum) SN test or ELISA
test.
In the event of a positive reaction to the SN test, the laboratory would
conduct the ELISA test; if the ELISA test is negative, the animal would be eligible to
remain in the centre. |
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13. |
In the event of a suspicious or positive reaction being reported during the
pre-entry isolation testing regime, the individual animal is to be removed from the group.
The remaining animals must not be released for entry into the production centre until
either the suspect animal has been confirmed negative or removed from isolation and the
negative status of the remaining animals reconfirmed for the disease in question. |
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14. |
Removal of donors from isolation either to enter the resident herd
following the test referred to above or to leave the centre, must be done only with the
permission of the accredited veterinarian. |
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15. |
A boar resident in a semen production centre for distribution in Canada
does not qualify to enter the isolation facility of a export semen production centre. Such
a resident boar must comply with the pre-entry requirements for export eligible semen (See
Export Eligible Semen section above) to enter the isolation facility of an export
eligible semen production centre. |
Owner's Use Only Semen
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16. |
Fresh semen-collected on the premises of a swine herd by
the owner for use in females present on the same premises-does not require the issuance of
any CFIA permit. Such fresh semen cannot be traded or sold and cannot leave the premises.
No record of semen collected for this purpose is required. |
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17. |
Fresh semen from swine-collected for any purpose other than for use on the
legal premises-must be collected in an approved semen collection centre. This does not
apply to semen collected for the purpose of evaluating the fertility of the donor, but
which is not intended to be inseminated into a female. |
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This module describes requirements for export eligible ovine
semen for domestic use and owner's use only, as well as the sire reference program entry
requirements. |
Ovine
Export Eligible Semen
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Requirements for rams intended for entry into a
semen collection centre to produce semen for export: |
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1. |
The flock of origin must not be subject to any quarantine and confirmed
free from scrapie, pulmonary adenomatosis, bluetongue, and other communicable diseases. To
the best of the veterinarian's knowledge and belief: a) during the previous
three years, pulmonary adenomatosis must not have been diagnosed in the flock of origin;
b) during the previous five years, scrapie must not have been diagnosed in the flock of
origin or in the flock in which the donor ram was born (if different from the flock of
origin); and,
c) the donor ram must not be the progeny of a sire or dam affected by scrapie. |
|
2. |
The animals must be subject to the following tests with negative results,
unless otherwise specified, within the 60 days before their entry into the isolation
section of the centre: i. Brucellosis - (serum) B.
melitensis, BPAT and CF (negative 1/5) - (serum) B.
ovis, CF.
ii. Leptospirosis - (serum) MAT - Serotypes L. pomona, and L. sejroe -
Rams with a test result of 1:100 or 1:200 must be retested after a minimum interval of 14
days and obtain a stable titre to be considered negative and eligible for entry into the
isolation facility of the centre.
iii. Johne's disease - (serum) AGID
iv. Bluetongue - (serum) ELISA
v. Maedi Visna - (serum) ELISA |
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3. |
A CFIA/ACIA 1634 Certificate of Health for Entry into a Semen
Production Centre certificate must accompany the animal to the isolation facility of the
centre. (Refer to paragraph 11 of 10.1 Artificial Insemination -
General Information for distribution details.) |
Domestic Use Semen
|
|
Requirements for rams intended for entry into a
semen collection centre to produce semen for distribution in Canada: Note:
If there is a request to collect sheep and goat semen for "Owner's use only,"
the procedure described in paragraphs 6 to 11 of 10.2 Artificial
Insemination - Bovine should be followed. |
|
4. |
The flock of origin must not be subject to any quarantine and confirmed
free from scrapie, pulmonary adenomatosis, bluetongue, and other communicable diseases. To
the best of the veterinarian's knowledge and belief: a) during the previous
three years, pulmonary adenomatosis must not have been diagnosed in the flock of origin;
b) during the previous five years, scrapie must not have been diagnosed in the flock of
origin or in the flock in which the donor ram was born (if different from the flock of
origin); and,
c) the donor ram must not be the progeny of a sire or dam affected by scrapie. |
|
5. |
The animals must be subject to the following tests with negative results
within the 60 days before their entry into the isolation section of the centre: i. Brucellosis - (serum) B. ovis,
CF
ii. Johne's disease - (serum) ELISA
iii. Maedi visna - (serum) ELISA |
|
6. |
A CFIA/ACIA 1634 certificate must accompany the animal to the
isolation facility of the centre. (Refer to paragraph 11 of Module
10.1 for distribution.) |
Sire Reference Programs
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The sire reference program must by approved and sanctioned by
the sheep breed association or producer group under whose auspices the semen is collected
and distributed. The health conditions under which the semen is collected must be fully
communicated to all producers who receive semen for the purpose of sire evaluation. |
|
7. |
All rams presented for entry in the approved isolation facility must
receive an injection of dihydrostreptomycin (25 mg per kg of body weight) or an alternate
antibiotic at the dosage recommended by the manufacturer, as a preventive measure against
leptospirosis. |
|
8. |
The donor animals or teasers should be placed in an isolation facility
approved by the district veterinarian and must not be exposed to any infection to which
they are susceptible from the date of pre-collection health testing until the collection
program is completed. |
|
9. |
After 30 days of isolation, the rams must be subject to the following tests
with negative results: i. Brucellosis - (serum) B.
ovis, CF
ii. Maedi Visna - (serum) ELISA |
|
10. |
A "Permit to collect semen" issued by the CFIA area office must
be issued before the semen is collected and distributed. A list of the producers
participating in the "Sire Reference Program" should be presented to the
district veterinarian. |
|
11. |
Semen collection and distribution may commence immediately after the test
results are available and complete records of the producers receiving the semen must be
maintained. |
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This module describes requirements for export eligible caprine
semen, domestic use and owner's use only. |
Caprine
Export Eligible Semen
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|
Requirements for bucks intended for entry into a
semen collection centre to produce semen for export: |
|
1. |
The flock of origin must not be subject to any quarantine and must be
confirmed free from scrapie, pulmonary adenomatosis, bluetongue, and other communicable
diseases. To the best of the veterinarian's knowledge and belief: a) during
the previous three years, pulmonary adenomatosis must not have been diagnosed in the flock
of origin;
b) during the previous five years, scrapie must not have been diagnosed in the flock of
origin or in the flock in which the donor ram was born (if different from the flock of
origin); and,
c) the donor ram must not be the progeny of a sire or dam affected by scrapie. |
|
2. |
The animals must be subject to the following tests with negative results,
unless otherwise specified, within the 60 days before entry into the isolation section of
the centre: i. Brucellosis - (serum) B. melitensis, BPAT and CF, negative at 1/5.
ii. Leptospirosis - (serum) MAT - Serotypes L. pomona, and L. sejroe -
Bucks with a test result of 1:100 or 1:200 must be retested after a minimum interval of 14
days and obtain a stable titre to be considered negative and eligible for entry into the
isolation facility of the centre.
iii. Johne's disease - (serum) AGID
iv. Bluetongue - (serum) ELISA
v. Caprine arthritis encephalitis - (serum) ELISA |
|
3. |
A CFIA/ACIA 1634 Certificate of Health for Entry into a Semen
Production Centre must accompany the animal to the isolation facility of the centre.
(Refer to paragraph 11 of 10.1 Artificial Insemination - General
Information for distribution details.) |
Domestic Use Semen
|
|
Requirements for bucks intended for entry into a
semen collection centre to produce semen for distribution in Canada: Note:
If there is a request to collect sheep and goat semen for "Owner's use only,"
the procedure described in paragraphs 6 to 11 of 10.2 Artificial
Insemination - Bovine should be followed. |
|
4. |
The flock of origin must not be subject to any quarantine and confirmed
free from scrapie, pulmonary adenomatosis, bluetongue, and other communicable diseases. To
the best of the veterinarian's knowledge and belief: a) during the previous
three years, pulmonary adenomatosis must not have been diagnosed in the flock of origin;
b) during the previous five years, scrapie must not have been diagnosed in the flock of
origin or in the flock in which the donor ram was born (if different from the flock of
origin); and,
c) the donor ram must not be the progeny of a sire or dam affected by scrapie. |
|
5. |
The animals must be subject to the following tests with negative results,
unless otherwise specified, within the 60 days before entry into the isolation section of
the centre: i. Johne's disease - (serum) AGID
ii. Caprine arthritis encephalitis - (serum) ELISA
Note: At the discretion of the centre management and the
agreement of the district veterinarian, bucks of positive serostatus may be permitted
entry into the centre provided that there are no animals of sero-negative status in the
centre during their entire residency and that the semen receptacles are clearly marked as
having originated from seropositive animals. |
|
6. |
A CFIA/ACIA 1634 certificate must accompany the animal to the
isolation facility of the centre. (Refer to paragraph 11 of Module
10.1 for distribution.) |
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This module describes the requirements for export eligible
cervine semen and for its distribution in Canada. Note: If there is
request to collect cervine semen for "Owner's use only," the procedure described
in paragraphs 6 to 11 of 10.2 Artificial Insemination - Bovine
should be followed. |
Cervine
Pre-Entry Health Requirements of Semen Donor Stags
|
|
1. |
The herd of origin of the donor stags must have been tested for
tuberculosis according to current Canadian standards as established in the Captive
Ungulate Policy. The herd of origin must not be subject to any quarantine and the
assessment of the general health of the herd must be conducted by the district
veterinarian. |
|
2. |
The herd of origin must be inspected and found free from clinical evidence
of infectious disease and insofar as can be determined, from any history of infectious
disease during the preceding 60 days. |
|
3. |
In the case of animals moving from the premises of origin to the approved
facility of a semen production centre, the donor stags should remain in isolation at the
premises of origin and must not be exposed to any infection to which they are susceptible
from the date of pre-entry health examination until entry into the designated isolation
facility. |
|
4. |
A CFIA/ACIA 1634 Certificate of Health for Entry into a Semen
Production Centre must accompany the animal to the isolation facility of the centre.
(Refer to paragraph 11 of 10.1 Artificial Insemination - Background
Information for distribution.) |
Isolation Health Requirements of Semen Donor Stags
|
|
The assessments of the isolation health status and isolation
testing of the donor stags are to be performed by a veterinarian authorized to perform
these functions as an accredited veterinarian with the exception of the
comparative cervical tuberculin test. Note: The isolation
facility must be approved by the district veterinarian before the donor stag is presented. |
|
5. |
All donor stags presented for entry as additions to the semen production
centre must undergo a minimum period of 30 days in an isolation facility maintained by the
centre; the isolation period may begin when all the animals have been assembled in the
isolation facility. |
|
6. |
An animal is to be considered eligible for entry into isolation only if a
certificate of health completed by an accredited veterinarian as per paragraph 4 above
accompanies the animal to the centre. |
|
7. |
The animal must be placed in an isolation facility which has been
pre-approved by the district veterinarian. Centres collecting cervine semen may be located
on premises on which other cervines are raised provided isolation guidelines are
maintained. |
|
8. |
While in the isolation facility, and after a minimum of 30 days in
isolation, the animals are tested for the following diseases with negative results, or
unless otherwise specified, as follows: i. Tuberculosis -
mid cervical intradermal tuberculin test using bovine purified protein derivative (PPD)
tuberculin or the comparative cervical test on known reactors to bovine
PPD tuberculin. The comparative cervical test is administered by CFIA staff under the same
conditions as outlined in the Captive Ungulate Program.
ii. Brucellosis - (serum) - BPAT; any reactor should be
confirmed negative by CF test negative at 1/5. |
|
9. |
If a suspicious or positive reaction is reported during the isolation
testing regime, the individual animal is to be removed from the group. The remaining
animals shall not be released for collection of semen until either the suspect animal has
been confirmed negative or has been removed from isolation and the negative status of the
remaining animals reconfirmed for the disease in question. Semen may be collected from the
non-reactors in the isolated group, but the semen must remain under CFIA control and be
released only after the disease freedom has been established by a negative retest. |
|
10. |
When tests results are known and negative, semen may be collected without
restriction. The donor stags may be collected in the isolation facility which is
considered to become a centre or move to a permanent semen collection centre. |
|
11. |
All semen collected from donor stags should be clearly labelled with the
identification of the donor animal, the species, the identity of the collection unit and
the date of collection of the semen. |
|
12. |
Any person or organisation who collects semen on a premises designated for
this purpose or on the premises of a herd owner using a mobile laboratory must be in
possession of a "Permit to Operate a Semen Production Centre" issued by the
National Coordinator, Artificial Insemination, Animal Health and Production Division,
Ottawa. In addition, every owner of premises on which semen is collected must be in
possession of a "Permit to collect semen" issued by the CFIA area office, unless
the owner of the premises is granted a "Permit to Operate a Semen Production
Centre." Cervine semen collected is eligible for export and distribution in Canada. |
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13. |
If donor stags are maintained in the semen collection centre or isolation
facility for a period of more than12 months, they should be retested for tuberculosis and
brucellosis every 12 months. |
Printer friendly PDF version |
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This module describes the requirements for bison semen for
exportation and for distribution in Canada. Note: If there is request
to collect cervine semen for "Owner use only," the procedure for "Owner's
Use Only Semen" mentioned in 10.2 Artificial Insemination -
Bovine should be followed. |
Bison
Export and Domestic Eligible Semen
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Pre-Entry Health Requirements of Semen Donor Bulls: |
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1. |
The herd of origin of the donor bulls must have been tested for
tuberculosis and brucellosis according to current Canadian standards. The herd of origin
must not be subject to any quarantine and the assessment of the general health of the herd
must be conducted by the district veterinarian. |
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2. |
The herd of origin must be inspected and found free from clinical evidence
of infectious disease and, insofar as can be determined, from history of infectious
disease during the preceding 60 days. |
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3. |
In the case of animals moving from the premises of origin to the approved
facility of a semen production centre, the donor bulls should remain in isolation at the
premises of origin and must not be exposed to any infection to which they are susceptible
from the date of pre-entry health examination until entry into the designated isolation
facility. |
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4. |
A certificate CFIA/ACIA 1634 Certificate of Health for Entry into a
Semen Production Centre must accompany the animal to the isolation facility of the
centre. (Refer to 10.1 Artificial Insemination - General Information
paragraph 11 for distribution.) |
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Isolation Health Requirements of Semen Donor Bulls: |
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Assessments of the isolation health status and isolation
testing of the donor bulls are to be performed by a veterinarian authorized to perform
these functions as an accredited veterinarian. Note: The isolation
facility must be approved by the district veterinarian before the donor bison is
presented. |
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5. |
All donor bulls presented for entry as additions to the semen production
centre must undergo a minimum period of 30 days in an isolation facility maintained by the
centre; the isolation period may begin when all animals have been assembled in the
isolation facility. |
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6. |
An animal is considered eligible for entry into isolation only if a
certificate of health completed by an accredited veterinarian as per paragraph 4 above
accompanies the animal to the centre. |
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7. |
The animal must be placed in an isolation facility which has been
pre-approved by the district veterinarian. Centres collecting bison semen may be located
on premises on which other bison are raised provided isolation guidelines are maintained. |
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8. |
All animals presented for entry as additions to the semen production centre
must receive an injection of dihydrostreptomycin (25 mg per kg of body weight) or an
alternate antibiotic at the dosage recommended by the manufacturer, as a preventive
measure against leptospirosis. |
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9. |
The initial tests of preputial samples for Campylobacter fetus subspecies
venerealis infection and trichomoniasis (Tritrichomonas foetus)
are conducted after at least 7 days in isolation. Preputial fluid examinations should not
be conducted until at least 7 days have elapsed following natural service or semen
collection by means of an artificial vagina. i. Campylobacter fetus
examination requires the use of a special Transport Enriched Medium (TEM) medium and
coordination with the laboratory when scheduling the submission of samples. Sample(s) of
preputial washing are to be subject to culture tests as follows:
- Animals less than 6 months of age or kept since that age only in a single sex group
before their entry into isolation should be tested once with negative results.
- Animals more than 6 months of age that could have been in contact with females before
entering isolation should be tested three times at weekly intervals with negative results.
ii. Trichomoniasis examination is by culture of preputial washing followed
by direct microscopic examination. The culture examination requires the use of a special Tritrichomonas Foetus Transport Medium (TFTM) medium or
acceptable alternate medium and coordination with the laboratory when scheduling the
submission of samples. Samples of preputial washing are to be subject to culture tests as
follows:
- Animals less than 6 months of age or kept since that age only in a single sex group
before their entry into isolation should be tested once with negative results.
- Animals more than 6 months of age that could have been in contact with females before
their entry into isolation should be tested three times at weekly intervals with negative
results.
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10. |
Semen may be collected from the donor immediately following each sampling
mentioned above, but must be labelled "Owner's Use Only" and may be released
immediately to the owner. |
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11. |
While in the isolation facility, and after a minimum of 30 days in
isolation, the donor animals are to be tested for the following diseases with negative
results unless otherwise specified: i. Brucellosis -
(serum) - BPAT, any reactor should be confirmed negative by CF test negative at 1/5.
ii. Tuberculosis - caudal intradermal tuberculin test
using bovine PPD tuberculin.
Note: Any reactions to these tests must be reported to the district
veterinarian. |
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12. |
If a suspicious or positive reaction is reported during the isolation
testing regime, the individual animal is to be removed from the group. The remaining
animals shall not be released for collection of semen until the suspect animal either has
been confirmed negative or has been removed from the isolation facility, and the negative
status of the remaining animals reconfirmed for the disease in question. |
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13. |
When tests results are known and negative, semen may be collected without
restriction. The donor bulls may be collected in the isolation facility which is
considered to become a centre or move to a permanent semen collection centre. |
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14. |
All semen collected from donor bulls should be clearly labelled with the
identification of the donor animal, the species, the identity of the collection unit, and
the date of collection of the semen. |
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15. |
Any person or organisation collecting semen on a premises designated for
this purpose or on the premises of a herd owner using a mobile laboratory must be in
possession of a "Permit to Operate a Semen Production Centre" issued by the
National Coordinator, Artificial Insemination, Animal Health and Production Division,
Ottawa. In addition, every owner of premises on which semen is collected must be in
possession of a "Permit to collect semen" issued by the CFIA area office, unless
the owner of the premises is granted a "Permit to Operate a Semen Production
Centre." Bison semen collected is eligible for export and distribution in Canada. |
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16. |
If donor bulls are maintained in the semen collection centre or isolation
facility for a period of more than 12 months, they should be retested for tuberculosis and
brucellosis every 12 months. |
