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Government of Canada Inventory of Ongoing Federal Food Safety and Nutrition Research: 2003Canadian Food Inspection AgencyTable of ContentsAbbreviated list of Canadian Food Inspection Agency projects (titles only) Canadian Food Inspection Agency project details Appendix C: Index by Pathogenic/ Toxic Agent, CFIA 2003 Projects Appendix D: Index by Commodity, CFIA 2003 Projects Appendix E: 2003 Index by Keyword, CFIA 2003 Projects IntroductionThe Inventory of Ongoing Federal Food Safety Research contains food safety projects that were being carried out by Agriculture and Agri-Food Canada, the Canadian Food Inspection Agency and Health Canada in 2003. This inventory is designed to provide scientists, science managers and top executives with a global view of the food safety research carried out in all federal institutions, in order to help identify gaps in research, develop a national research strategy, and stimulate collaboration. The inventory was designed to be easy to use, with a short initial core section intended for decision and policy makers consisting of a list of project titles and a summary table, followed by appendices in which the projects are described in more detail for use by scientists. The inventory was also designed to be easy to update. Each project has been given a unique alpha-numerical ID Project Number, and this number will never change until the project is completed and removed from the inventory. As an example, project number CFIA0215 is the fifteenth Canadian Food Inspection Agency project entered in the inventory in the year 2002. The projects are presented following the pattern: Project number Project title Abbreviated list of projects (titles only)Canadian Food Inspection AgencyCFIA0201 Adaptation and evaluation of a gene probe method and a multiplex polymerase chain reaction method for the detection and enumeration of Vibrio parahaemolyticus CFIA0202 Improved test capability for veterinary drug residues in dairy, eggs and honey CFIA0204 Development/ implementation of analytical methods for the multi-residue determination of chloramphenicol, florfenicol and thiamphenicol in honey CFIA0206 Development/ implementation of analytical method for the determination of cephalosporins in milk CFIA0207 Development/ implementation of analytical method for the determination of coccidiostats in egg and egg products CFIA0210 Development/ implementation of analytical method for the determination of dipyrone, etodolac and metabolites in milk CFIA0212 Development/ implementation of analytical method for the determination of tetracyclines in egg and egg products CFIA0213 Development/ implementation of an expansion in the scope of pesticides analyzed in the pesticide multiresidue methods of analysis of fruit and vegetables CFIA0214 Development/ implementation of analytical method for the determination of abamectin in fruit and vegetable commodities CFIA0215 Development/ implementation of analytical method for the determination of imidacloprid in honey and its subsequent extension for the determination of imidacloprid in fruit and vegetable commodities CFIA0218 Development, optimization, validation and transfer of nucleic acid sequence-based amplification and polymerase chain reaction techniques for the detection and epidemiological characterization of food-borne viruses in foods (shellfish, fruits, vegetables, meat products) and livestock CFIA0220 Study of protein expression profiles of Listeria monocytogenes using two-dimensional electrophoresis for strain identification, discrimination of the relative virulence potential and epidemiological applications CFIA0224 Low density deoxyribonucleic acid and antibody arrays for the detection and identification of foodborne pathogens CFIA0235 Methods for the isolation and detection of Cryptosporidium in apple cider CFIA0236 Establishment of accurate and rapid tests for food- and water-borne parasites: Cyclospora, Giardia, and Toxoplasma CFIA0237 Standardization and validation of immunohistochemical and PCR assays for Cysticercus bovis CFIA0243 Development of analytical methodology for the determination of synthetic pyrethroid residues in tissue of slaughtered animals and in milk and dairy products CFIA0247 Improved test capability for banned substances in food of animal origin CFIA0248 Development and implementation of analytical methods for the determination of carbadox and five other feed additives (spiramycin, tylosin, virginiamycin, bacitracin and olaquindox) banned by the European Union for use as antimicrobial and growth promotants in food animal production CFIA0251 Development and implementation of multi-residue analytical methods for the determination of Beta-lactams and phenicols in animal derived foods CFIA0252 Development/ implementation of analytical methods for macrolide and aminoglycoside antibiotics in foods of animal originals CFIA0253 Development/ implementation of analytical methods for the detection of prednisone and other corticosteroids in meat and meat products CFIA0257 Evaluation of high through-put, rapid tests for bovine spongiform encephalopathy (BSE) CFIA0258 Enhancement and implementation of the Canada Database of Animal Parasites CFIA0301 Mass spectrometric verification of the endectocides doramectin, moxidectin, abamectin, ivermectin, and eprinomectin in milk CFIA0302 Development/ implementation of a multi-residue confirmatory method for the determination of Beta-lactams or penicillins in eggs and milk using LC MS/MS CFIA0303 Development/ implementation of a multi-residue confirmatory method for the determination of macrolides in eggs and milk using LC MS/MS CFIA0304 Development/ implementation of analytical methods for the detection of specific fungicides in fruit and vegetable commodities CFIA0305 Development of HPLC/MS methodology for the determination of azaspiracids in shellfish CFIA0306 Development of HPLC/MS methodology for the determination of nitrofuran metabolites in aquacultured products CFIA0307 Carbon monoxide detection in carbon monoxide-treated fish flesh CFIA0308 Functional foods containing fish oils: Correlating sensory analysis with volatile odour compounds CFIA0309 Development and optimization of a nucleic acid sequence-based amplification (NASBA) "real-time" platform for the detection of hepatitis and Norwalk-like viruses in foods CFIA0310 Development, optimization and validation of a new PCR and culture procedures for the detection and isolation of Shigella spp. in fresh produce CFIA0311 Development, optimization and validation of molecular methods for the detection of enteric viruses in bottled water and berries CFIA0312 Confirmatory tests to corroborate milk allergen detection in various food matrix CFIA0313 Detection of emerging zoonotic enteric viruses potentially transmitted through livestock and food CFIA0314 Evaluation of the suitability of a low density array method using a universal sample preparation procedure for the detection of foodborne viruses using DNA and RNA enteric viruses as models CFIA0315 Evaluation and validation of the NASBA) amplification approach for the detection of enteroviruses in food samples CFIA0316 Rapid detection and reduction of Salmonella Typhimurium DT 104 in poultry using bacteriophage CFIA0317 Development of a novel, rapid and inexpensive method for epidemiological analysis of Listeria monocytogenes and Escherichia coli strains isolated from clinical cases and contaminated food during disease outbreaks CFIA0318 Evaluation of postmortem diagnostic tests for confirmation of chronic wasting disease in clinically affected and preclinical elk and deer CFIA0319 Development of a bioassay to detect infectivity in tissues and body fluids of elk and deer naturally infected with chronic wasting disease CFIA0320 Preliminary investigation of host response patterns in chronic wasting disease and scrapie as disease markers for preclinical diagnostic tests CFIA0321 Development of ultrasensitive immuno-PCR and enzyme immuno-fluorescence techniques for food testing: detection of bacterial toxins and quantitation of peanut allergen in foods CFIA0322 Validation of ELISA for antemortem diagnosis of Cysticercus bovis-infected cattle CFIA0323 Improved test capability for hormones and other growth promoting substances in urine and tissues CFIA0324 Development/ implementation of a multi-residue analytical method to determine beta-lactams in honey using liquid chromatography/ electrospray ionization tandem mass spectrometry CFIA0325 Development/ implementation of analytical method to determine aminoglycosides in honey CFIA0326 Development/ implementation of analytical method for the determination of tetracyclines in egg and egg products Comprehensive list of projects (all fields)Canadian Food Inspection Agency project detailsCFIA0201 Adaptation and evaluation of a gene probe method and a multiplex polymerase chain reaction method for the detection and enumeration of Vibrio parahaemolyticus Project leader: Enrico A Buenaventura, Canadian Food Inspection Agency-Burnaby, buenaventura@inspection.gc.ca Starting date: 2001/04 Collaboration: Canadian Food Inspection Agency-Sidney Description: Even though both pathogenic and non-pathogenic strains of Vibrio parahaemolyticus occur naturally in the environment and in molluscan shellfish including oysters, the current testing methods do not distinguish between these strains. A multiplex detection system for Vibrio parahaemolyticus will eliminate the need to perform individual tests for each of the individual genotypes, reducing the time and cost involved in confirmatory testing. This study will adapt and evaluate new methods to detect and enumerate Vibrio parahaemolyticus. Project Identification: BB0101 CFIA0202 Improved test capability for veterinary drug residues in dairy, eggs and honey Project leader: Dugane Quon, Canadian Food Inspection Agency-Calgary, quond@inspection.gc.ca Starting date: 2000/04 Description: A need for better analysis for veterinary drug residues in honey, eggs and dairy products was noted in the European Community audits of Canada's Residue Control Programs for animal-derived foods in September 2000. This study will enhance Canadian Food Inspection Agency test capabilities to detect and analyze listed compounds in the residue control program. Project Identification: CF0101 CFIA0204 Development/ implementation of analytical methods for the multi-residue determination of chloramphenicol, florfenicol and thiamphenicol in honey Project leader: David Durden, Canadian Food Inspection Agency-Calgary, durdend@inspection.gc.ca Starting date: 2002/04 Description: There is a concern that the chloramphenicol and other phenicol compounds may be present in honey. Objectives are to adapt and validate a residue method for phenicols (chloramphenicol, florfenicol, thiamphenicol) in honey, based upon the present chloramphenicol method in eggs and milk; develop a more sensitive analytical method module for phenicols and their metabolites, for screening or confirmation. Project Identification: CF0202 CFIA0206 Development/ implementation of analytical method for the determination of cephalosporins in milk Project leader: David Durden, Canadian Food Inspection Agency-Calgary, durdend@inspection.gc.ca Starting date: 2002/04 Description: Cephalosporins, as part of the large group of Beta-lactam antibiotics , are widely used in agriculture, especially for treatment of mastitis in lactating cattle. Canadian Food Inspection Agency has methods for analysing ceftiofur residues in tissue. This method, however, has not been transferred to other matrices such as milk or eggs. Following the European Union audit report of 2000, several other cephalosporins, and possibly their metabolites, were suggested to be added to the list of food residues. The objectives are to adapt and validate a residue method for cephalosporins in milk. Project Identification: CF0204 CFIA0207 Development/ implementation of analytical method for the determination of coccidiostats in egg and egg products Project leader: Dugane Quon, Canadian Food Inspection Agency-Calgary, quond@inspection.gc.ca Starting date: 2002/04 Description: Two coccidiostats, nicarbazin and robenidine, are prohibited as veterinary medical products for food producing animals in the European Community. Canadian products must be tested to meet international levels for export purposes. This study will expand on the capabilities of the Canadian Food Inspection Agency's Canada National Chemical Residue Monitoring Program to analyze for coccidiostats in eggs and egg products. Project Identification: CF0205 CFIA0210 Development/ implementation of analytical method for the determination of dipyrone, etodolac and metabolites in milk Project leader: Gail White, Canadian Food Inspection Agency-Calgary, whitegail@inspection.gc.ca Starting date: 2002/04 Description: The United States Food and Drug Administration has banned dipyrone for human use, and removed it from its list of approved veterinary medications. The drug is also banned in Canada and many Western European countries. Canadian Food Inspection Agency has a method for detecting dipyrone and metabolites in bovine and porcine muscle. Since the milk matrix is somewhat more complex than the tissue matrix, a new extraction procedure will have to be developed. Objectives are to adapt and validate a residue method for dipyrone, its metabolites and etodolac in milk. Project Identification: CF0208 CFIA0212 Development/ implementation of analytical method for the determination of tetracyclines in egg and egg products Project leader: David Durden, Calgary, durdend@inspection.gc.ca Starting date: 2002/04 Description: The Canadian Food Inspection Agency has quantified tetracyclines in a variety of food stuff, and test methods have been developed in both Calgary and Saskatoon. A method has been developed at the Calgary laboratory for tetracycline residues (oxytetracycline, tetracycline, chlortetracycline) in honey. It is proposed to extend this method to eggs and egg products. Objectives are to adapt and validate a residue method for tetracyclines in eggs and egg products and to add marker compounds to the residue method. Project Identification: CF0210 CFIA0213 Development/ implementation of an expansion in the scope of pesticides analyzed in the pesticide multi-residue methods of analysis of fruit and vegetables Project leader: Donna Grant, Canadian Food Inspection Agency-Calgary, grantd@inspection.gc.ca Starting date: 2002/04 Description: The pesticide multi-residue method in Canadian Food Inspection Agency Calgary will screen for as many as 265 different pesticides in fruit and vegetable matrices. Canada must expand the number of pesticides screened to harmonize with the United States Department of Agriculture, and Food and Drug Administration. This study will expand the scope of pesticides currently available in the pesticide multi-residue analysis of fruit and vegetable commodities. Project Identification: CF0211 CFIA0214 Development/ implementation of analytical method for the determination of abamectin in fruit and vegetable commodities Project leader: Chris Tam, Canadian Food Inspection Agency-Calgary, tamchris@inspection.gc.ca Starting date: 2002/04 Description: To harmonize with the United States Department of Agriculture, and Food and Drug Administration, there is a need to update the scope of pesticides being monitored in Canada to include those monitored by American government departments. This study will adapt and validate a residue method to determine abamectin in fruit and vegetables. Project Identification: CF0212 CFIA0215 Development/ implementation of analytical method for the determination of imidacloprid in honey and its subsequent extension for the determination of imidacloprid in fruit and vegetable commodities Project leader: Chris Tam, Canadian Food Inspection Agency-Calgary, tamchris@inspection.gc.ca Starting date: 2002/04 Description: A major initiative of the Canadian Food Inspection Agency's Calgary laboratory is to expand the pesticide multi-residue method, which currently screens for as many as 265 different pesticides in fruit and vegetable matrices. However, some pesticides would not be good candidates for inclusion in this method. Hence, alternate methods are required for these pesticides. This project will address those pesticides identified as being of high priority for chemical residue monitoring. Objectives are to adapt and validate a residue method to determine imidacloprid in honey, and extend and validate the method to determine imidacloprid in fruit and vegetables. Project Identification: CF0213 CFIA0217b Development of sensory reference and training kits for fishing industry Project leader: D.G. McLachlan, Canadian Food Inspection Agency-Dartmouth, mclachlang@inspection.gc.ca Starting date: 1998/04 Description: This study will investigate the volatile chemical makeup of several key fish and fish products, using gas chromatographic techniques to gain the information necessary to develop and produce standardized olfactory calibration reference kits. The standard calibration reference kits will approximate the various sensory odours and intensities associated with fish, from fresh caught to advanced decomposition. Project Identification: FI9801 CFIA0218 Development, optimization, validation and transfer of nucleic acid sequence-based amplification and polymerase chain reaction techniques for the detection and epidemiological characterization of food-borne viruses in foods (shellfish, fruits, vegetables, meat products) and livestock Project leader: Carole Simard, Canadian Food Inspection Agency-St. Hyacinthe, simardc@inspection.gc.ca Starting date: 2000/04 Collaboration: Canadian Food Inspection Agency-Ottawa (Carling), Agriculture and Agri-Food Canada: Food Research and Development Centre, Health Canada: Bureau of Microbial Hazards - Food Directorate, Université Laval; Intitute National de la Recherche Agronomique -Institut Armand Frappier Description: Assess the significance of foods in the transmission of enteric viruses, as foods are likely to contain only low levels of viruses, adequate concentration, purification and detection methods must be developed. This study will investigate the development, optimization, validation and transfer of rapid molecular techniques to detect, and epidemiologically characterize, food-borne viruses in foods and livestock. Project Identification: H0102 CFIA0220 Study of protein expression profiles of Listeria monocytogenes using two-dimensional electrophoresis for strain identification, discrimination of the relative virulence potential and epidemiological applications Project leader: Min Lin, Canadian Food Inspection Agency-Ottawa (Fallowfield), linm@inspection.gc.ca Starting date: 2002/04 Description: Whereas listeriosis is a rare human illness, it remains a leading cause of mortality from a food-borne pathogen. A rapid detection method for Listeria monocytogenes in perishable products, including meat, poultry, processed egg and dairy products, and products which have an inherently high background level of bacteria, is required. This study examines a novel typing methodology for differentiating Listeria monocytogenes strains at an extremely high resolution, and for assessing their virulence potentials. Project Identification: NBR0219 CFIA0224 Low density DNA and antibody arrays for the detection and identification of food-borne pathogens Project leader: Burton W. Blais, Canadian Food Inspection Agency-Ottawa (Carling), bblais@inspection.gc.ca Starting date: 2002/09 Description: The development of a simple, rapid and inexpensive low density array technology based on the use of macroporous hydrophobic cloth (polyester cloth) as a solid support for the immobilization of DNA and antibody arrays is proposed. Objectives are to develop simple DNA arrays capable of identifying several key virulence, toxin and antibiotic resistance genes found in a variety bacteria targeted by Canadian Food Inspection Agency food microbiology testing programs; and an antibody array for use in serotyping Salmonella isolates. Project Identification: OFL0201 CFIA0235 Methods for the isolation and detection of Cryptosporidium in apple cider Project leader: Sarah Parker, Canadian Food Inspection Agency-Saskatoon, sparker@inspection.gc.ca Starting date: 2002/04 Description: Cryptosporidium is a water-borne protozoan increasingly recognised as an important human pathogen. The ability to detect Cryptosporidium in apple juice is required, both in the context of investigations during an outbreak situation, and during periods of surveillance or routine testing of apple juice for the presence of pathogens. The objective of this study is to identify detection methods for Cryptosporidium with the most promise for accuracy and rapid results. Project Identification: SA0202 CFIA0236 Establishment of accurate and rapid tests for food- and water-borne parasites: Cyclospora, Giardia, and Toxoplasma Project leader: Alvin Gajadhar, Canadian Food Inspection Agency-Saskatoon, agajadhar@inspection.gc.ca Starting date: 2002/04 Description: Objectives of this study are to identify accurate and rapid techniques for detection of the food- and water-borne parasites Cyclospora, Toxoplasma and Giardia; establish standardized protocols for the diagnostic tests identified for Cyclospora, Toxoplasma and Giardia; and determine the detection limits and analytical specificity for these diagnostic tests with purified parasites. Project Identification: SA0203 CFIA0237 Standardization and validation of immunohistochemical and polymerase chain reaction assays for Cysticercus bovis Project leader: Brad Scandrett, Canadian Food Inspection Agency-Saskatoon, bscandrett@inspection.gc.ca Starting date: 2002/04 Description: Canadian Food Inspection Agency has recently developed an immunohistochemical and a polymerase chain reaction assay for postmortem diagnosis of Cysticercus bovis, the intermediate stage of the human tapeworm Taenia saginata, in cattle. Preliminary data suggest that these assays are more sensitive and specific than histological evaluation. This study will compare the three assays and provide evidence in support of, or against, adoption of the immunohistochemical and PCR assays for routine diagnostic use. Project Identification: SA0205 CFIA0243 Development of analytical methodology for the determination of synthetic pyrethroid residues in tissue of slaughtered animals and in milk and dairy products Project leader: Christine Akre, Canadian Food Inspection Agency-Saskatoon, akrec@inspection.gc.ca Starting date: 2000/04 Description: Synthetic pyrethroids are among the most widely used classes of insecticides for plant protection. Persistent residues have been reported in livestock, and routine monitoring programs in other countries have found detectable residues of various pyrethroids in fat. This study will adapt, validate and test a multi-residue method for pyrethroid residues in fat to monitor for residues in domestic and imported meat. This project selected a method that has been used by United States Department of Agriculture, Food Safety and Inspection Service, for testing. An assessment of the method in its original form is under way. Project Identification: SF0001 CFIA0247 Improved test capability for banned substances in food of animal origin Project leader: Joe Boison, Canadian Food Inspection Agency-Saskatoon, jboison@inspection.gc.ca Starting date: 2000/04 Collaboration: Western College of Veterinary Medicine Description: Continuing improvement of test capability for banned substances in food of animal origin, this project will cover four distinct groups of drugs that are either banned in Canada or by our trading partners, or have not been approved for use in food animals by Health Canada. This study will investigate Canadian Food Inspection Agency test capabilities equivalent to the European Union, to detect and analyze banned substances in the residue control program. Project Identification: SF0103 CFIA0248 Development and implementation of analytical methods for the determination of carbadox and five other feed additives (spriamycin, tylosin, virginiamycin, bacitracin and olaquindox) banned by the European Union for use as antimicrobial and growth promotants (AGPs) in food animal production Project leader: Joe Boison, Canadian Food Inspection Agency-Saskatoon, jboison@inspection.gc.ca Starting date: 2000/04 Collaboration: Western College of Veterinary Medicine Description: This study will adapt published methods to determine the feed additives virginiamycin and olaquindox, and develop a method to determine bacitracin residues in animal tissues. The enhanced test capability will afford an increased level of protection to Canadian consumers by contributing to Canadian Food Inspection Agency's residue control program. Project Identification: SF0104 CFIA0251 Development and implementation of multi-residue analytical methods for the determination of Beta-lactams and phenicols in animal derived foods Project leader: Craig Salisbury, Canadian Food Inspection Agency-Saskatoon, csalisbury@inspection.gc.ca Starting date: 2002/04 Description: This project will focus on the development of methods to determine residues of phenicols (thiamphenicol and the recently-registered florfenicol) and Beta-lactams(amoxicillin, ampicillin, and cloxacillin) in animal tissues, dairy products and egg products and on the application of these methods to a pilot survey of field samples from the antibiotic survey program. Project Identification: SF0201 CFIA0252 Development/ implementation of analytical methods for macrolide and aminoglycoside antibiotics in foods of animal origin Project leader: Les Dickson, Canadian Food Inspection Agency-Saskatoon, dicksonl@inspection.gc.ca Starting date: 2002/04 Description: A series of multi-residue methods to determine selected antibiotic compounds (macrolides and aminoglycosides) in animal tissues, milk and dairy products, and eggs and egg products will be developed or adapted from literature methods. The validated methods will be incorporated into diagnostic programs to address the need for monitoring these residues in domestic and imported meat, and products of animal origin. Project Identification: SF0202 CFIA0253 Development/ implementation of analytical methods for the detection of prednisone and other corticosteroids in meat and meat products Project leader: Rick Fedeniuk, Canadian Food Inspection Agency-Saskatoon, rfedeniuk@inspection.gc.ca Starting date: 2002/04 Collaboration: Canadian Food Inspection Agency: Winnipeg Laboratory (Foreign Animal Diseases) Description: Objectives are to develop and validate an analytical method(s) to determine prednisone (a synthetic glucocorticosteroid that has been used as a growth-promoter in animal production and can mask signs of disease in animals prior to slaughter) and its metabolites to 500 parts per trillion in meat and meat products; develop liquid chromatography mass spectrometry/mass spectrometry confirmatory ability for prednisone and relevant metabolites; and determine current state of prednisone use in food markets. Project Identification: SF0203 CFIA0257 Evaluation of high through-put, rapid tests for bovine spongiform encephalopathy Project leader: Stefanie Czub, Canadian Food Inspection Agency-Saskatoon, Czubs@inspection.gc.ca Starting date: 2002/04 Description: The purpose of the study is to prepare the implementation of a high through-put bovine spongiform encephalopathy assay. The situation might occur where rapid and high through-put tests are required to increase the level of monitoring of Canadian cattle, especially should a positive animal be detected in Canada or the United States by immunohistochemistry or histopathology. Project Identification: W0206 CFIA0258 Enhancement and implementation of the Canada Database of Animal Parasites Project leader: Lydden Polley, Canadian Food Inspection Agency – Saskatoon, lpolley@inspection.gc.ca Starting date: 2002/04 Collaboration: Western College of Veterinary Medicine Description: The Canada Database of Animal Parasites contains records of the occurrence of twenty important helminth and protozoan parasites assembled from the published literature as far back as possible, and from the veterinary diagnostic laboratories. The focus this year will be on continuing to update the database from both publications and diagnostic laboratories (including data validation), developing the parasite profiles or providing links to pre-existing sources of information and using available mechanisms to make CDAP more accessible within the CFIA. Project identification: SA0201 CFIA0301 Mass spectrometric verification of the endectocides doramectin, moxidectin, abamectin, ivermectin, and eprinomectin in milk Project leader: David A. Durden, Canadian Food Inspection Agency – Calgary, durdend@inspection.gc.ca Starting date: 2003/01 Description: This project is aimed at developing an LC MS/MS method for the confirmation of endectocide antiparasitic drugs in milk. This method is intended to provide verification for milk samples which prove positive for the above endectocides. Project identification: CF0302 CFIA0302 Development/ implementation of a multi-residue confirmatory method for the determination of Beta-lactams or penicillins in eggs and milk using LC MS/MS Project leader: Jiang Wang, Canadian Food Inspection Agency – Calgary, wangj@inspection.gc.ca Starting date: 2003/04 Collaboration: CFIA Saskatoon Laboratory - Veterinary Drug Residues Description: This study is designed to develop LC MS/MS methodology to confirm the identity and concentration of Beta-lactams in shell egg and raw milk samples. Project identification: CF0303 CFIA0303 Development/ implementation of a multi-residue confirmatory method for the determination of macrolides in eggs and milk using LC MS/MS Project leader: Jiang Wang, Canadian Food Inspection Agency – Calgary, wangj@inspection.gc.ca Starting date: 2003/04 Collaboration: CFIA Saskatoon Laboratory – Veterinary Drug Residues Description: Macrolides in eggs and milk are currently being screened using the Charm II test in CFIA. Any positive samples need to have the presence of macrolides confirmed. The present study is to develop LC MS/MS methodology to confirm the identity of macrolides in shell egg and raw milk samples. Project identification: CF0304 CFIA0304 Development/ implementation of analytical methods for the detection of specific fungicides in fruit and vegetable commodities Project leader: Donna Grant, CFIA – Calgary, grantd@inspection.gc.ca Starting date: 2003/04 Description: Analytical methods for residues from specific fungicides in fruit and vegetable commodities will be developed, validated and implemented into ongoing monitoring and surveillance regulatory programs within the CFIA Calgary Laboratory. Project identification: CF0305 CFIA0305 Development of HPLC/MS methodology for the determination of azaspiracids in shellfish Project leader: Garth Burns, Canadian Food Inspection Agency – Dartmouth, burnsbg@inspection.gc.ca Starting date: 2003/04 Description: This project is designed to develop methodology to analyse for toxins, hopefully within a multi-toxin analysis that will include DSP (diarrhetic shellfish poisoning )and pectenotoxins. The method will allow for monitoring of the toxins to provide early warning of contamination or to monitor outbreaks. The method will be presented to the scientific community upon completion. Project identification: D0301 CFIA0306 Development of HPLC/MS methodology for the determination of nitrofuran metabolites in aquacultured products Project leader: Garth Burns, Canadian Food Inspection Agency – Dartmouth, burnsbg@inspection.gc.ca Starting date: 2003/04 Description: Nitrofuran antibiotics have proven to be a problem because their metabolites persist in host tissue, and as a result of their promotion of mutagenicity, genotoxicity and chromosome damage. These antibiotics are used to combat bacterial infections in aquacultured products in a number of countries, and as a result their residues can be expected to be found in aquacultured product being imported into Canada. It is proposed that a method in use by the RIKILT Institute in the Netherlands be modified for use within this laboratory. Once the method is validated it will be transferred to other CFIA laboratories and will be available to industry, academia and the scientific community. It is expected that the methodology will eventually be used in a monitoring program. Project identification: D0302 CFIA0307 Carbon monoxide detection in CO treated fish flesh Project leader: Dave McLachlan, Canadian Food Inspection Agency – Dartmouth, mclachlandg@inspection.gc.ca Starting date: 2003/04 Description: In recent years carbon monoxide (CO) and "tasteless smoke" (filtered smoke with a high percentage of CO present) have been administered by fish processors in several off-shore countries to several fish species to preserve and enhance flesh colour. At present, carbon monoxide treatment is an unapproved process in Canada, therefore CO treated product is not permitted to be imported into Canada. This study is to develop a quantitative assay which can be conducted in any laboratory with the use of simple plastic/ glassware and a relatively inexpensive ($500) digital carbon monoxide specific monitor (sensitivity 0 – 1 parts per million to 999 parts per million). The analysis would allow for the effective monitoring of suspect product entering the country, with a minimum cost in materials and analysis time. Project identification: D0303 CFIA0308 Functional foods containing fish oils: Correlating sensory analysis with volatile odour compounds Project leader: Dave McLachlan, Canadian Food Inspection Agency – Dartmouth, mclachlandg@inspection.gc.ca Starting date: 2003/04 Collaboration: industry partner Description: A primary objective in this proposal is to utilize the CFIA expertise in sensory analysis to identify marker compounds which are characteristic odours caused by oil rancidity or oxidation, as well as due to interactions with the food matrix. Results will be used to establish sensory product standard guidelines for regulatory use to ensure consumer safety when used in functional foods. Project identification: D0304 CFIA0309 Development and optimization of a NASBA "real-time" platform for the detection of hepatitis and Norwalk-like viruses (NLVs) in foods Project leader: Yvon-Louis Trottier, Canadian Food Inspection Agency – St. Hyacinthe, trottieryl@inspection.gc.ca Starting date: 2003/04 Collaboration: Agriculture and Agri-Food Canada: Food Research and Development Centre, St. Hyacinthe; industry partner Description: This project aims to increase the detection sensitivity of food-borne viruses (hepatitis A and Norwalk-like viruses) and to develop a quantitative assay for the detection of hepatitis A and Norwalk-like Viruses (NLVs) in fruits and vegetables (spiked blueberries and lettuce). Project identification: H0302 CFIA0310 Development, optimization and validation of a new PCR and culture procedures for the detection and isolation of Shigella species in fresh produce Project leader: Yvon-Louis Trottier, Canadian Food Inspection Agency – St. Hyacinthe, trottieryl@inspection.gc.ca Starting date: 2003/04 Collaboration: Health Canada: Food Directorate, Ottawa Description: Food-borne diseases associated with Shigella species are becoming an increasing risk to human health in developed countries such as United States and Canada. Minimally processed foods, such as fresh vegetables and salads, have been increasingly implicated in outbreaks. This study will focus on the optimization and the validation of the current PCR procedure for the detection of Shigella in fresh produce, used by the United States Food and Drug Administration’s Centre for Food Safety and Applied Nutrition. This methodology has proven to be effective for the detection of all Shigella species and Entero-Invasive Escherichia coli (EIEC). This project is being accomplished in collaboration with Health Canada scientists in order to comply with the requirements of the Compendium of Analytical Methods. Project identification: H0303 CFIA0311 Development, optimization and validation of molecular methods for the detection of enteric viruses in bottled water and berries Project leader: Yvon-Louis Trottier, Canadian Food Inspection Agency – St. Hyacinthe, trottieryl@inspection.gc.ca Starting date: 2003/04 Collaboration: Agriculture and Agri-Food Canada: Food Research and Development Centre, St. Hyacinthe Description: Recent food-borne outbreaks of acute viral gastroenteritis in Canada and the United States have occurred through commodities such as water, fresh fruits and vegetables (strawberries, raspberries, salads) and shellfish. Norovirus (Norwalk-like viruses) and hepatitis A viruses have been responsible for the majority of these outbreaks. Although several molecular detection methods have been published, only a handful of methodologies are available to extract the viruses from selected food matrices (such as berries) and bottled water. The objective of this study is to provide an efficient extraction method to remove the viral particles from bottled water and berries, removing inhibitors and interfering substances, followed by a concentration step and detection using optimized molecular detection methods. Project identification: H0304 CFIA0312 Confirmatory tests to corroborate milk allergen detection in various food matrix Project leader: Philippe Raymond, Canadian Food Inspection Agency – St. Hyacinthe, raymondp@inspection.gc.ca Starting date: 2003/04 Collaboration: Canadian Food Inspection Agency – Longueuil; Health Canada: Allergen Method Development Laboratory (Ottawa) Description: Objectives are to develop and evaluate different confirmatory methodologies for the detection of milk allergens adapted to the food matrix composition. Cookies, chocolate bars, frozen yogurt are representative "milk-free" food where hidden milk allergens were reported. Researchers will further purify and characterize milk proteins and develop new milk protein reagents and antibodies to improve our testing capacity. These approaches will then be compared to the standard milk allergen ELISA for the different food matrix types. Researchers expect to select the most appropriate confirmatory test for hidden milk allergens and the information gathered from testing the different matrix types would be highly valuable or directly transferable to other allergens in similar matrices. Project identification: H0305 CFIA0313 Detection of emerging zoonotic enteric viruses potentially transmitted through livestock and food Project leader: Yvon-Louis Trottier, Canadian Food Inspection Agency – St. Hyacinthe, trottieryl@inspection.gc.ca Starting date: 2003/04 Collaboration: Agriculture and Agri-Food Canada: Food Research and Development Centre, St. Hyacinthe Description: The main objective of this project is to detect emerging zoonotic enteric viruses in livestock, i.e. cattle, sheep and swine. Noroviruses (Norwalk, Norwalk-like), the TT virus, the bovine Newbury Agent-2 and the hepatitis E virus will be targeted. Molecular methods with primers specific to the targeted viruses will be used to detect the presence of the viruses in the feces of selected animals in abattoirs and on farms. The genomic portions amplified using molecular methods will be sequenced and compared with existing databases so that Canadian sequences can be compared with those from other countries. Project identification: H0306 CFIA0314 Evaluation of the suitability of a low density array method using a universal sample preparation procedure for the detection of food-borne viruses using DNA and RNA enteric viruses as models Project leader: Yvon-Louis Trottier, Canadian Food Inspection Agency – St. Hyacinthe, trottieryl@inspection.gc.ca Starting date: 2004/01 Collaboration: Agriculture and Agri-Food Canada: Food Research and Development Centre, St. Hyacinthe; industry partner Description: Food-borne viruses are an increasing concern in Canada and the United States. Although viruses do not multiply in foods, they are, nonetheless, resistant to environmental storage conditions and common practices for controlling bacterial contamination. Since only a few viral particles can become a risk to the consumer, one of the main challenges facing diagnostic laboratories is to be able to concentrate these viral particles from the suspected foods, in order to increase the probability of detection by molecular methods and eliminate inhibitors that may interfere with the amplification process. The objective of our project is to evaluate a "universal extraction/ concentration" procedure applicable to both viruses and bacteria from various spiked food matrices (a "one step multi-pathogen" procedure). Rotavirus type A and Adenovirus 40-41 have been selected as a model for RNA virus and DNA virus respectively. Project identification: H0307 CFIA0315 Evaluation and validation of the NASBA amplification approach for the detection of enteroviruses in food samples Project leader: Carole Simard, Canadian Food Inspection Agency – St. Hyacinthe, simardc@inspection.gc.ca Starting date: 2003/02 Collaboration: University of Montreal: Faculty of Veterinary Medicine; Université Laval : Département des sciences des aliments et de nutrition; Agriculture and Agri-Food Canada: Food Research and Development Centre Description: The objective of this project is to compare various approaches for the extraction and concentration of enteroviruses from various food samples, as a preliminary step to detection by molecular methods. We will compare various approaches for the recovery of hepatitis A virus and rotavirus from various food samples, characterize the extraction-amplification method developed, and validate the method by the analysis of samples of seafood, fruits and vegetables. Project identification: H0311 CFIA0316 Rapid detection and reduction of Salmonella Typhimurium DT 104 in poultry using bacteriophage Project leader: Jiewen Guan, Canadian Food Inspection Agency – Ottawa (Fallowfield), guanj@inspection.gc.ca Starting date: 2003/04 Collaboration: Canadian Institute for Food Safety Research; Department of Food Science, University of Guelph; industry Description: In this study, we will investigate the development of a rapid detection method for screening Salmonella Typhimurium DT 104. The method is expected to be sensitive and specific, and will incorporate selective enrichment and immunomagnetic separation. The entire detection procedure is estimated to be completed within 36 hours, and will provide a rapid alternative to time consuming and labour intensive conventional culture methods. In addition, we will also investigate the feasibility of using phage to reduce the load of Salmonella Typhimurium DT 104 in experimentally infected chickens. The study will provide information on the potential use of phage as an alternative to antibiotics for controlling multi-drug resistant pathogens in agriculture. Project identification: NAV0320 CFIA0317 Development of a novel, rapid and inexpensive method for epidemiological analysis of Listeria monocytogenes and Escherichia coli strains isolated from clinical cases and contaminated food during disease outbreaks Project leader: Min Lin, Canadian Food Inspection Agency – Ottawa (Fallowfield), linm@inspection.gc.ca Starting date: 2003/04 Collaboration: Queen’s University: Centre for Water and the Environment. Description: This study is designed to develop a novel, rapid, simple and inexpensive tool for epidemiological analysis of two important food-borne pathogens Listeria monocytogenes and Escherichia coli O157:H7. The tool will be used by food safety labs to rapidly trace back the sources responsible for outbreaks and sporadic cases in epidemiological studies. It will also allow for the accurate detection, identification and quantification of those Listeria monocytogenes strains and Escherichia coli O157:H7 in the food chains that pose the greatest risk to human health. Project identification: NBR0320 CFIA0318 Evaluation of postmortem diagnostic tests for confirmation of chronic wasting disease in clinically affected and preclinical elk and deer Project leader: Arumuga Balachandran, Canadian Food Inspection Agency – Ottawa (Fallowfield), balachandrana@inspection.gc.ca Starting date: 2003/04 Collaboration: Animal Disease Research Unit (ADRU), United States Department of Agriculture, Pullman, Washington; United States Department of Agriculture, Ames, Iowa; Colorado State University Description: Any eradication program for disease in livestock is dependant on the validity of the diagnostic criteria. The critical issue in relation to chronic wasting disease is the deficit of information on possible correlations between various diagnostic tests available. Commercial firms in Europe are developing based tests for detection of the scrapie prion protein (PrPSc) in brain tissue of TSE suspected animals. The United States Department of Agriculture and CFIA are collaborating with these groups to evaluate the tests as large-scale screening and surveillance tools. Project identification: NTSE0101 CFIA0319 Development of a bioassay to detect infectivity in tissues and body fluids of elk and deer naturally infected with chronic wasting disease Project leader: James Algire, Canadian Food Inspection Agency – Ottawa (Fallowfield), jalgire@inspection.gc.ca Starting date: 2003/04 Description: The main aim of this project is to establish a bioassay system to measure the infectivity in the various tissues and products of chronic wasting disease infected animals, and to aid in the diagnosis of chronic wasting disease in subclinically infected (carrier animals) not showing signs of disease. Project identification: NTSE0204 CFIA0320 Preliminary investigation of host response patterns in chronic wasting disease and scrapie as disease markers for preclinical diagnostic tests Project leader: Hongsheng Huang, Canadian Food Inspection Agency – Ottawa (Fallowfield), huangho@inspection.gc.ca Starting date: 2003/04
Description: While the methods to diagnose transmissible spongiform encephalopathies (TSEs) including chronic wasting disease and scrapie have improved tremendously over the past decade, early detection in living animals remains problematic. The objective of this study is to catalogue a series of blood-borne markers associated with TSEs with a view to recognize a specific response pattern to discriminate diseased animals from healthy animals. Presence of a specific response pattern (or lack of it) at specific time points during incubation and clinical disease may assist in recognizing carrier animals. Project identification: NTSE0304 CFIA0321 Development of ultrasensitive immuno-PCR and enzyme immuno-fluorescence techniques for food testing: detection of bacterial toxins and quantitation of peanut allergen in foods Project leader: Burton W. Blais, Canadian Food Inspection Agency – Ottawa (Carling), bblais@inspection.gc.ca Starting date: 2003/04 Collaboration: Health Canada: Bureau of Microbial Hazards; Health Canada: Guelph, Ontario; Canadian Food Inspection Agency-Ottawa (Fallowfield); industry Description: This project addresses the development and evaluation of ultrasensitive fluorescence-based assay systems as novel technology platforms for the detection of priority analytes in foods. To demonstrate the broad applicability of these technologies this study will focus on the development of applications targeting analytes for the detection of bacterial toxins and food allergens. Test systems will be developed and evaluated for the detection of the priority toxins botulinum neurotoxin (BoNT, type E) and verotoxin (VT2), and for the detection and quantification of peanut protein in foods. The performance characteristics of the assay system showing the greatest promise will be further evaluated to assess its suitability. This assay format will also be adapted to the detection of VT2. Project Identification: OLC0301 CFIA0322 Validation of ELISA for antemortem diagnosis of Cysticercus bovis-infected cattle Project leader: Dele Ogunremi, Canadian Food Inspection Agency – Saskatoon, dogunremi@inspection.gc.ca Starting date: 2003/04 Description: Cattle infected with the tapeworm cyst Cysticercus bovis are difficult to detect at meat inspection because most infected animals harbour only a few cysts. Sensitive antemortem detection based on the detection of antibodies in bovine serum should help in minimizing the costs of managing infected herds by providing a tool that will enable the focussing of intervention strategies on infected cattle only. The aim of this project is to standardize and validate an antibody detection ELISA for the diagnosis of Cysticercus bovis. Banked serum samples obtained from past investigations of both experimental and natural infected cattle will be used for analysis, as will negative bovine serum samples. Cattle infected with other helminths will be included as controls. It is expected that at the end of the study, test parameters will be available to support the use of the test for antemortem detection of Cysticercus bovis-infected cattle. Project identification: SA0304 CFIA0323 Improved test capability for hormones and other growth promoting substances in urine and tissues Project leader: Jim MacNeil, Canadian Food Inspection Agency – Saskatoon, jmacneil@inspection.gc.ca Starting date: 2001/04 Description: This project was established to develop and implement tests to expand the scope of testing for growth promoting substances. The tests will meet method validation requirements of revised European Union directives. Methods under investigation include liquid chromatography, gas-chromatography (GC/MS) and LC MS/MS. Project Identification: SF0101 CFIA0324 Development/ implementation of a multi-residue analytical method to determine beta-lactams in honey using liquid chromatography/ electrospray ionization tandem mass spectrometry Project leader:Jiang Wang, Canadian Food Inspection Agency – Calgary, wangj@inspection.gc.ca Starting date: 2002/04 Description: The beta-lactam antibiotics are used to treat infectious human and animal diseases and to enhance growth and yield in agriculture. Maximum residue limits for beta-lactam antibiotics drug residues in some food commodities, such as milk and eggs, have been established by Health Canada, the European Union and the United States Food and Drug Administration’s Center for Veterinary Medicine. This project will adapt and validate a multi-residue analytical method for beta-lactams in honey and transfer the method to the analytical testing staff at the Calgary Laboratory. Project Identification: CF0201 CFIA0325 Development/ implementation of analytical method to determine aminoglycosides in honey Project leader: Dugane Quon: Canadian Food Inspection Agency – Calgary, quond@inspection.gc.ca Starting date: 2002/04 Description: CFIA has the capability to screen for aminoglycosides (streptomycin residues) in eggs and milk. There is a concern that streptomycin residues may be present in honey, particularly in imported honey. This project will allow the CFIA to expand its capability to analyze and confirm the presence of streptomycin residues in honey. Project Identification: CF0203 CFIA0326 Development/ implementation of analytical method for the determination of tetracyclines in egg and egg products Project leader: David A. Durden, Canadian Food Inspection Agency – Calgary, durdend@inspection.gc.ca Starting date: 2002/04 Description: One of the major groups of antibiotics is that of tetracyclines. The CFIA has quantified tetracyclines in a variety of food stuffs, and a method has been developed in Calgary for tetracycline residues (oxytetracycline, tetracycline, chlortetracycline) in honey. Objectives are to adapt and validate a residue method for tetracyclines in eggs and egg products by LC/MS, to add marker compounds to the LC/MS residue method and to transfer the methods to Calgary’s analytical testing staff. Project Identification: CF0209 |
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