Laboratory Biosafety Guidelines
3rd Edition 2004
PDF Format (479 KB) 
Cat. No. H39-4/49-2004E-PDF
ISBN 0-662-37723-0
Table of Contents
Editorial Committee and Acknowledgements
Abbreviations
Chapter 1
Introduction
Chapter 2
Biological Safety
2.1 Risk Groups
2.2 Containment Levels
2.3 Risk Assessment
2.4 Health and Medical Surveillance
2.5 Management of Biological Safety
2.6 Biosecurity
Chapter 3
Handling Infectious Substances
3.1 Operational Practices for Laboratories
3.1.1 General Practices
3.1.2 Containment Level 2
3.1.3 Containment Level 3
3.1.4 Containment Level 4
Chapter 4
Laboratory Design and Physical Requirements
4.1 Matrix 1: Laboratory Location and Access
4.2 Matrix 2: Surface Finishes and Casework
4.3 Matrix 3: Heating, Ventilation and Air Conditioning
4.4 Matrix 4: Containment Perimeter
4.5 Matrix 5: Laboratory Services
Chapter 5
Commissioning, Certification and Recertification for CL3 and CL4 Laboratories
5.1 Introduction
5.1.1 Commissioning
5.1.2 Certification
5.1.3 Recertification
5.2 Room Integrity
5.2.1 Matrix 6: Room Integrity
5.2.2 Room Pressure Decay Testing
5.3 Air Handling Systems
5.3.1 Matrix 7: Air Handling Systems
5.4 Matrix 8: Laboratory Equipment and Services
Chapter 6
Large Scale Production of Microorganisms
6.1 Introduction
6.2 Scope
6.3 Operational Practices and Physical Requirements
6.3.1 Containment Level 1 Large Scale
6.3.2 Containment Level 2 Large Scale
6.3.3 Containment Level 3 Large Scale
Chapter 7
Program Specific Guidelines
7.1 Laboratory animals
7.1.1 General Requirements
7.1.2 Non-human Primates
7.2 Recombinant DNA and Genetic Manipulation
7.3 Cell Lines
7.3.1 Risk Assessment
7.3.2 Contamination with Infectious Agents
7.3.3 Self-Self Experiments
Chapter 8
Decontamination
8.1 Introduction
8.2 Autoclaves
8.3 Chemical Disinfection
8.4 Gaseous Decontamination of Rooms
8.5 Liquid Effluent Treatment Systems
8.6 Irradiation
8.7 Incineration
8.8 New Technologies
Chapter 9
Biological Safety Cabinets
9.1 Introduction
9.2 Classes and Characteristics of Biological Safety Cabinets
9.3 Installation and Certification
9.4 Use of the Cabinet
Chapter 10
Regulatory Aspects for Handling Infectious Substances
10.1 Importation and Transfer of Human Pathogens
10.2 Export of Pathogens
10.3 Transportation
10.4 Importation, Transfer, and Containment of Animal Pathogens
Please note that this document was published by Health Canada prior to the announcement of the establishment of the Public Health Agency of Canada on September 24, 2004. Any reference to Health Canada should be assumed to be to the Public Health Agency of Canada.
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