The applicant must ensure that the trial seed and/or plant material are
transported in clearly identified, secure containers and are kept separate from other seed
and/or plant material.
Surplus transgenic trees from the trial must be clearly labelled and kept
in a secure greenhouse facility or must be destroyed by mechanical means, heating or
burning. Composting of this material is not an acceptable destruction method.
The trial term will be limited to six (6) years from the date of
commencement.
Seeding, transplanting and site maintenance machinery and equipment must
be cleaned at the trial site to prevent dispersal of plant material.
In the case of accidental release, recoverable seeds or seedlings must be
collected and destroyed, the site must be marked and monitored, and the PBO notified
immediately. Plants from unrecoverable seed or seedlings must be mechanically or
chemically destroyed.
Two guard rows must be composed of non-transformed poplar (Populus deltoides x trichocarpa) producing no or very few
suckers.
The trial material (including the guard rows) will be separated by a
distance of at least 15 meters from other trees of the same related species. The trial
site and isolation distance must be monitored, at a minimum, twice a week during the
period of flowering and budburst and, at a minimum, monthly during the growing season of
the trail period to ensure that all suckers, precocious inflorescences and trees of same
or related species that are not part of the trial are removed and destroyed.
During the trial period, all precocious inflorescences (if any) must be
removed each year before the anthesis to prevent pollen dissemination and seed setting.
Records must be kept of the date and number of flowering catkins removed from each genetic
line.
Measurements from permanent surrounding landmarks must be provided for
precise location of the site. Markers must also be placed at all corners of the trial site
to identify the confined field trial boundaries. The markers must be obvious, identifiable
and in place for the growing seasons of both the trial and the post-harvest restriction
period.
Global Positioning System (GPS) coordinates must be taken precisely at
all corners of each trial site. The GPS coordinates of each confined research field trial
site location must be submitted to the PBO within 7 days after planting.
If a chemical treatment is used on the crop other then those used for
general agronomic management, a sign must be posted at the access to the trial indicating
the date and time of spraying as well as the time until safe entry. This
condition is intended to protect the health and safety of the CFIA inspection staff.
No plant material from these trials may enter the human food or livestock
feed chain unless approved by Health Canada or the Feeds Section, CFIA, respectively.
Harvesting machinery and equipment will be cleaned of all residual plant
material at the trial site prior to being moved to other locations. Plant material
harvested, that is not to be retained, must be destroyed by burning, autoclaving, or
burial to a depth of one metre. Composting of this material is not an acceptable
destruction method.
Harvested seed and/or propagable plant material from the confined
research field trial may only be retained if requested in the application and authorized
by the PBO. Any harvested seed and/or plant material must be clearly labelled, securely
transported, and stored separately from other seed and/or plant material.
Applicants must provide the PBO in writing within 15 working days after
harvest with information on:
- quantity of seed and/or plant material harvested at the trial sites
- date(s) of harvest
- quantity of seed and/or plant material disposed of
- location, method and date of disposal
- quantity of seed and/or plant material retained and stored
- storage location and method
Disposal of plant material (propagable and/or non-propagable) includes
harvested plant material as well as residual plant material on the trial site.
If a trial is destroyed prior to harvest applicants must provide the PBO in
writing within 15 working days after destruction with information on the trial's growth
stage at the time of destruction, as well as the date and method of destruction.
A detailed trial log book must be kept. Records of the confined research
field trial, including current season and post-harvest site monitoring, activities related
to the trial site compliance, cleaning of machinery and transportation, disposition and
storage of all harvested seed and plant material, must be maintained by the applicant and
made available to the CFIA upon request. A report summarizing the completed trial and
experimental data, including any amendments to the original protocol, must also be made
available to the CFIA upon request. Detailed records requirements can be found in section
3.8 of Regulatory Directive 2000-07.
The trees (including guard rows) must be cut down at the end of the trial
period. Plant matter remaining at the end of the trial must be destroyed. Stumps and root
systems must either be mechanically destroyed on site or removed and destroyed. The trial
site must be tilled and any developing suckers after tillage must be destroyed.
The trial site must not be used to grow poplar trees from the date of
termination of the trial until no suckers are observed for three (3) consecutive years.
The site must be monitored, at a minimum, monthly during the post-harvest growing seasons
to ensure that all volunteer plants, suckers and related species are removed and
destroyed.
Applicants must notify the PBO in writing of crop species planted on
trial sites for each year the sites are subject to post-harvest restriction. This
notification must be received every year by June 15.