Supplement to All Methods that Detect Salmonella
Appendix J
Health Products and Food Branch - Ottawa
Supplement to all Salmonella Methods
November 2003
Microbiological Methods Committee
Microbiology Evaluation Division
Bureau of Microbial Hazards, Food Directorate,
Health Products and Food Branch, Health Canada
Postal Locator: 2204A1
Ottawa, Ontario K1A 0L2
Don_Warburton@hc-sc.gc.ca
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1. APPLICATION
The following information is offered as a supplement to all
methods that detect Salmonella in food, food ingredients and
environmental samples and should be used with these methods.
The purpose of this supplement is to provide additional
information (such as source(s) of materials, critical steps,
confirmation steps, or helpful hints etc.).
5. MATERIALS AND SPECIAL EQUIPMENT
The medium listed below (item 20) is commercially available
and is to be prepared and sterilized according to the
manufacture's instructions. See also Appendix G of Volume
2 in this Compendium.
19) Incubators or water baths capable of maintaining
35°C (Non-selective enrichment broths, plates and SC
broth) and 42.5°C (TBG and RVS broths)
20) RVS broth (Oxoid 866)
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NOTE: RVS broth is listed as RV broth
in Appendix G.
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6. PROCEDURE
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NOTE: Due to safety and environmental
issues, SC broth can be replaced with RVS broth in all
methods used for the detection of Salmonella in foods,
food ingredients and environmental samples.
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SC broth must be used (using the conditions described below)
when analysing guar gum and when the presence of Salmonella
Typhi is suspected, such as in outbreaks or for imports from
tropical countries where S. Typhi is prevalent. In general,
it is recommended that SC be used in the investigation of
food borne outbreaks.
6.3 Selective Enrichment
6.3.1 If substituting RVS for SC broth, transfer 0.1 mL
and 1.0 mL of the preenrichment culture into 9 mL of RVS
and TBG broths, respectively. If using SC follow MFHPB-20.
6.3.2 Incubate RVS and TBG broths for 24 ± 2 h at
42.5°C.
6.4 Selective Plating
6.4.1 Follow MFHPB-20 for TBG and RVS broths. If using SC
broth, streaking must be done using sterile disposable loops
and in a fume hood or a biohazard hood.
8. PREPARATION OF MEDIA
8.1 RVS Broth
Use Oxoid (866) or see Appendix G.
9. DISPOSAL OF SELENITE CYSTINE (SC) BROTH AND PRODUCTS
CONTAINING SC
NOTE: All products containing SC
MUST be disposed of through a waste
management facility. This includes ALL
dry product and reconstituted product. Any product that
has been contaminated must be autoclaved to
sterilization (using a properly ventilated system)
prior to disposal.
UNDER NO CIRCUMSTANCES IS ANY SC PRODUCT TO BE
DISPOSED OF THROUGH THE DRAINAGE OR SEWER
SYSTEM.
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