A List of the Seventeen Studies and Publications Submitted by the European
Commission in Relation to Hormonal Growth Promoters Used in Beef Cattle
1. Presence of Estrogen in Meat (delivery of samples)
No publications to be reviewed.
2. Hormones as Growth Promoters: Genotoxicity and Mutagenicity
of Zeranol &Trenbolone
"Genotoxic potential of xenobiotic growth promoters and their metabolites"
(APMIS 109:89-95, 2001)
3. Metabolic Pathways of Estrogens as Steroidal Growth Promoting
Agents
"Estrogenic activity of estradiol and its metabolites in the ER-CALUX
assay with human T47D breast cells"
(APMIS 109: 101-107, 2001)
4. Metabolites of Melengestrol Acetate, Trenbolone Acetate & Zeranol
in Bovine & Humans
"Metabolism of melengestrol acetate and trenbolone";
(publication foreseen )
5. Application of Anabolic Agents to Food-producing Animals - Health Risks Through Disregard of Requirements of Good Veterinary Practice
1)"Detection of melengestrol acetate residues in plasma and edible
tissues of heifers"
(The Veterinary Quarterly 21: 154-158, 1999 )
2) "Detection of anabolic residues in misplaced implantation sites
in cattle"
(Journal of AOAC International 83(4); 809-819, 2000)
3) "Suppression of androstenone in entire male pigs by anabolic
preparations"
(Livestock Production Science- 69: 139-144, 2001)
4) "A sensitive enzyme immunoassay (EIA) for the determination
of Melengestrol acetate (MGA) in adiposeand muscle tissues"
(Food Additives and Contaminants 18(4):285-291, 2001)
5) "Characterisation of the affinity of different anabolics and
synthetic hormones to the human androgen receptor, human sex hormone binding
globulin and to the bovine progestin receptor"
(APMIS 108: 838-846, 2000)
6) "Dose-dependent effects of melengestrol acetate (MGA) on plasma
levels of estradiol, progesterone and luteinizing hormone in cycling heifers
and influences on oestrogen residues in edible tissues"
(APMIS 108: 847-854, 2000)
7) "Hormone contents in peripheral tissues after correct and off-label
use of growth promoting hormones in cattle: Effect of the implant preparations
Finaplix-H®, Ralgro®, Synovex-H® and Synovex Plus®"
(APMIS 109: 53-65, 2001)
8) "Tissue-specific expression pattern of estrogen receptors (ER):
Quantification of Erα and ERβ mRNA with real-time RT-PCR"
(APMIS 109: 345-355, 2001)
6. Analysis of 500 Samples for the ppresence of Growth Promoters
"Hormones found in meat samples from regular controls within the
EU and from US imports"
(Chemical awareness; issue 9, July5th 2000)
7. Analysis of 500 Samples for the Presence of Growth Promoters
1) "Ultra trace detection of a wide range of anabolic steroids
in meat by gas chromatography coupled to mass spectrometry"
(Journal of Chromatography A, 867: 219-233, 2000)
2) "Le contrôle des anabolisants dans la viande"
(The survey of anabolic agents in meat)
(Annales de Toxicologie Analytique, vol.XII, no.1,2000)
8. Comparison of Assay Methods
1) "Frequency and molecular analysis of hprt mutations
induced by estradiol in Chinese hamster V79 cells"
(International Journal of Oncology 17:1141-1149, 2000)
2) "Estrogens as endogenous genotoxic agents-DNA adducts and mutations"
(Monographs, JNCI 27: 75-93, 2000)
3) "Tissue-specific synthesis and oxidative metabolism of estrogens"
(Monographs, JNCI 27: 95-112, 2000)
4) "Induction of uterine adenocarcinoma in CD-1 mice by catechol
estrogens"
(Cancer Research 60: 235-237, January 15, 2000 )
5) "Genotoxicity of the steroidal estrogens estrone and estradiol:
possible mechanism of uterine and mammary cancer development"
(Human Reproduction Update, 7 (3): 273-281,2001)
9. Bioassay of Estrogenic/Anti-Estrogenic Compounds
"Assessment of oestrogenic potency of chemicals used as growth
promoter by in-vitro methods"
(Human Reproduction 16: 1030-1036, 2001)
10. Interaction of Xenobiotics with Sex Hormone Binding Globulin:
Impact on Endogenous Steroid Transport, Bioavailability, Mechanism of
Action
(The scientist has not yet indicated the name of journal and publication
date.)
11. Reproductive Sequelae of Developmental Exposure of Rabbits
to Trenbolone, Zeranol & MGA; Emphasis on Differential & Neoplastic
transformation of Germ Cells
(Publication was anticipated by the end 2001 - not yet available.)
12. Long Term Effects in Children to estrogenized Meat
"Accidental gynecomastia in children"
(APMIS 109(suppl.103): 203-209, 2001)
13. Androgen Exposures in Utero, Risk of Breast Cancer
"A study of opposite-sexed twins"
(Journal of The National Cancer Institute, JNCI, volume 93, issue
1;60-62, 3.1.2001)
14. Endocrine Disrupting Activity of Anabolic Steroids Used
in Cattle
1) "Characterisation of the affinity of different anabolics and
synthetic hormones to the human androgen receptor, human sex hormone binding
globulin and the bovine progestin receptor"
(APMIS 108: 838-846, 2000)
2) "The fate of trenbolone acetate and melengestrol acetate after
application as growth promotants in cattle - environmental studies"
(Environmental Health Perspectives- in preparation)
15. Screening Water Samples for Estrogenic & Androgenic
Anabolic Chemicals
scientist has not yet indicated name of journal and publication
date.
Some results can be found in APMIS 109(suppl.103): 551-556, 2001
General discussion on "Existing guidelines for the use of meat
hormones and other food additives in Europe and USA "
16. Endocrine Disrupting Effects of Cattle Farm Effluent on
Environmental Sentinel Species
"A re-examination of variation associated with environmentally
stressed organisms"
(Human Reproduction update vol.7 (no.3): 265-272, 2001)
17. Human Cells Exposed to the Estrogenic Compound Zeranol
"Oestrogenic potencies of zeranol, oestradiol, diethylstilboestrol,
Bishpenol-A, and genistein: Implications for exposure assessment of potential
endocrine disrupters"
(Human Reproduction 16: 1037-1045, 2001)
Other publications in relation to the subject:
Intrauterine exposure to diethylstilbestrol: long-term effects in
humans
(APMIS 108: 793-804, 2000)
Getting the problem of endocrine disruption into focus: the need
for a pause for thought
(APMIS 108: 805-13, 2000)
Human exposure to endocrine disrupters: standardisation of a marker
of estrogenic exposure in adipose tissue
(APMIS vol.109:issue 3, 2001)
Threshold analysis of selected dose-response for endocrine active
chemicals
(APMIS vol.109:issue 3, 2001)
Biochemistry and physiology of anabolic hormones used for improvement
of meat production
(APMIS 109; 1-8, 2001)
Possible health impact of phytoestrogens and xenoestrogens in food
(APMIS vol.109:161 84; 2001)
Possible health impact of animal oestrogens in food
(Human Reproduction Update, vol.7, no.3 pp. 340-355, 2001
Assessment of estradiol and its metabolites in meat
(APMIS 109: 32-8, 2001)
Hormones in meat: different approaches in the EU and in the USA
(APMIS 109, suppl. 103, 357-64, 2001)
Hormones and Endocrine Disrupters in Food and Water: Possible Impact
on Human Health
(Forty-three peer-reviewed papers and discussions from an
international workshop held at the University Hospital Rigshospitalet,
Copenhagen , Denmark, May 27- 30, 2000 (Reprints of APMIS, Supplementum
no. 103, vol 109, 2001) )
Endnotes:
APMIS - Acta Pathologica, Microbiologica et Immunologica Scandinavia
AOAC - Scientific Association Dedicated To Analytical Excellence
JNCI - Journal of the National Cancer Institute
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