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MATERIAL SAFETY DATA SHEET - INFECTIOUS SUBSTANCES SECTION I - INFECTIOUS AGENT NAME: Escherichia coli, enteropathogenic SYNONYM OR CROSS REFERENCE: EPEC, attaching and effacing E. coli (AEEC), enteroadherant E. coli (EAEC), acute diarrhea, infantile diarrheal disease CHARACTERISTICS: Gram negative rod; motile, aerobic; non - enterotoxin producing and non - enteroinvasive; serogroups possess an antigenic adherence factor (bundle-forming pili BFP); serotyping to determine somatic and flagellar antigens SECTION II - HEALTH HAZARD PATHOGENICITY: Intestinal disease accompanied by watery diarrhea, fever, cramps and vomiting; bloody stool in some cases; serious disease in infants EPIDEMIOLOGY: Associated with outbreaks of acute diarrheal disease in newborn nurseries; occurs sporadically as well; EPEC no longer an important cause of infant diarrhea in North America and Europe; major agent of infant diarrhea in many developing countries (South America, South Africa, Asia), infants < 1 year old HOST RANGE: Humans, especially infants < 2 years, most mammals (livestock) INFECTIOUS DOSE: highly infectious for infants, does unknown, presumably low; Adults by Ingestion - 100,000,000 organisms to 10,000,000,000 organisms (108 to 1010) MODE OF TRANSMISSION: Fecal contamination of food, water or fomites; fecal-oral spread; may be spread to infants during delivery or by contaminated hands; poor hygiene and poor sanitation INCUBATION PERIOD: 12-72 hours (9-12 hrs in adult volunteer studies) COMMUNICABILITY: Communicable period not known, but presumably for the duration of fecal excretion, which may be prolonged several weeks SECTION III - DISSEMINATION RESERVOIR: Infected persons, often asymptomatic; animals ZOONOSIS: Yes - direct or indirect contact with infected animals and wastes VECTORS: None SECTION IV - VIABILITY DRUG SUSCEPTIBILITY: Susceptible to ampicillin, TMP-SMX SUSCEPTIBILITY TO DISINFECTANTS: Susceptible to many disinfectants - 1% sodium hypochlorite, 70% ethanol, iodines, phenolics; glutaraldehyde, formaldehyde PHYSICAL INACTIVATION: Inactivated by moist heat (121° C for at least 15 min) and dry heat 160-170° C for at least 1 hour SURVIVAL OUTSIDE HOST: Dust 4 to 27 days; feces - up to 84 days; fingertip - 45 min; soil - up to 84 days SECTION V - MEDICAL SURVEILLANCE: Monitor for symptoms; confirm bacteriologically, serologically FIRST AID/TREATMENT: Electrolyte fluid therapy (oral or IV); antibiotics may be administered in very severe cases IMMUNIZATION: None PROPHYLAXIS: Not usually administered SECTION VI - LABORATORY HAZARDS LABORATORY-ACQUIRED INFECTIONS: 2 reported cases of laboratory infections with E. coli SOURCES/SPECIMENS: Feces PRIMARY HAZARDS: Ingestion SPECIAL HAZARDS: None SECTION VII - RECOMMENDED PRECAUTIONS CONTAINMENT REQUIREMENTS: Biosafety level 2 practices, containment equipment and facilities for activities involving cultures and infected clinical materials PROTECTIVE CLOTHING: Laboratory coat; gloves when contact with infectious materials is unavoidable OTHER PRECAUTIONS: Good personal hygiene and frequent handwashing SECTION VIII - HANDLING INFORMATION SPILLS: Allow aerosols to settle; wearing protective clothing, gently cover spill with absorbent paper towel and apply 1% sodium hypochlorite, starting at perimeter and working towards the centre; allow sufficient contact time (30 min) before clean up DISPOSAL: Decontaminate before disposal; steam sterilization, chemical disinfection STORAGE: In sealed containers that are appropriately labelled SECTION IX - MISCELLANEOUS INFORMATION Date prepared: January, 2001 Prepared by: Office of Laboratory Security, PHAC Although the information, opinions and recommendations contained in this Material Safety Data Sheet are compiled from sources believed to be reliable, we accept no responsibility for the accuracy, sufficiency, or reliability or for any loss or injury resulting from the use of the information. Newly discovered hazards are frequent and this information may not be completely up to date. Copyright © [Material Safety Data Sheets - Index]
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Last Updated: 2001-09-27 | ![]() |