Ultrasensitive detection and imaging methods are enabling technologies for the development of a new generation of medical diagnostics. Advances in this area require the molecular scale detection and imaging of individual biomolecules in the complex environment of a living cell. SIMS Biomolecular Sensing and Imaging Group develops and applies tools for molecular sensing and imaging and for understanding cellular processes. Current expertise focuses on the following 3 areas:
Molecular Sensing at Chemically Modified Semiconductor Surfaces We have expertise in methods for the chemical modification of semiconductor surfaces and the characterization of their properties. Methods for the controlled covalent attachment of a range of organic molecules (including biomolecules) to the silicon surface have been demonstrated, employing both wet chemistry and vacuum based approaches. The suitability of these modified surfaces for a range of molecular sensing and other applications are being explored. Studies aimed at understanding the fundamental mechanisms of the attachment reactions and the nature of electronic coupling at organic/semiconductor interfaces are also being carried out.
High Resolution Imaging of Membrane Organization Understanding the organization of membranes requires a range of advanced imaging tools that provide high sensitivity (single molecule) and nanometer scale resolution and that can probe the dynamics of the interactions of biomolecules. We employ combinations of scanned probe microscopy and fluorescence techniques to study model supported membranes and cellular membranes. These studies are directed towards understanding the molecular basis for the organization of membranes into small microdomains and how these microdomains influence protein clustering and the formation of signaling platforms in cellular membranes.
Development of New tools At the forefront of modern biological research is the need for new tools to understand fundamental processes. Our research involves developing new tools and technologies for understanding cellular processes including molecular networks, signal transduction, and protein assemblies. We develop chemical probes for functional genomics and proteomics and for the microfabrication of nanoscale devices.
