Pathogenesis

Markus Czub

Head, Pathogenesis Unit, Zoonotic Diseases and Special Pathogens Programme, National Microbiology Laboratories, PHAC, Winnipeg.
Adjunct Professor, Dept. of Medical Microbiology, University of Manitoba

Degrees:

• DVM (Berlin, Germany, Approbation 1984)
• Dr. med. vet. (Dissertation in Veterinary Virology, Veterinary School, Giessen, Germany, 1988)
• Dr. med. habil. (Habilitation in Virology, Medical School, Würzburg, Germany, 1998)
• DVM (College of Veterinarians of Ontario, Public service license, Canada, 2001)

Telephone: 204-789-6037   
Lab: 204-789-6029 / 204-789-5014
Fax : 204-789-2140
Email:  markus_czub@phac-aspc.gc.ca

Research Interests:

Pathogenesis, prevention and epidemiology of high containment and emerging viruses, such as Nipah, Crimean-Congo-Hemorrhagic-Fever, and SARS-Corona virus. Development of diagnostic tools and procedures.

Selected projects:

1. Paramyxoviruses (Nipah, Hendra, Canine Distemper virus): a) Study of molecular mechanisms employed for viral entry into cells. b) Design and development of inhibitors of specific steps of viral replication. c) Identification of determinants of neurovirulence.

2. Crimean-Congo-Hemorrhagic-Fever virus: Study of virus transmission between various mammalian hosts via tick vectors.

Recent Publications:

Hansen, R., Czub, S., Werder, E., Herold, J., Gosztonyi, G., Gelderblom, H.R., Schimmer, S., Mazgareanu, S., ter Meulen, V., and Czub, M.: Abundant Defective Viral Particles Budding from Microglia in the Course of Retroviral Spongiform Encephalopathy. J. Virol. 74, 1775-1780, 2000.

Czub, S., Koutsilieri, E., Sopper, S., Czub, M., Stahl-Hennig, C., Müller, J.G., Pedersen, V., Gsell, W., Heeney, J.L., Gerlach, M., Gosztonyi, G., Riederer, P., ter Meulen V.: Enhancement of central nervous system pathology in early simian immunodeficiency virus infection by dopaminergic drugs. Acta Neuropathol. (Berl) 101, 85-91, 2001.

Hansen, R., Sauder, C., Czub, S., Bachmann, E., Schimmer, S., Hegyui, A., and Czub, M.: Activation of Microglia is Dispensable for the Induction of Rat Retroviral Spongiform Encephalopathy. J. NeuroVirol. 7, 501-510, 2001.

Marra MA, Jones SJ, Astell CR, Holt RA, Brooks-Wilson A, Butterfield YS, Khattra J, Asano JK, Barber SA, Chan SY, Cloutier A, Coughlin SM, Freeman D, Girn N, Griffith OL, Leach SR, Mayo M, McDonald H, Montgomery SB, Pandoh PK, Petrescu AS, Robertson AG, Schein JE, Siddiqui A, Smailus DE, Stott JM, Yang GS, Plummer F, Andonov A, Artsob H, Bastien N, Bernard K, Booth TF, Bowness D, Czub M, Drebot M, Fernando L, Flick R, Garbutt M, Gray M, Grolla A, Jones S, Feldmann H, Meyers A, Kabani A, Li Y, Normand S, Stroher U, Tipples GA, Tyler S, Vogrig R, Ward D, Watson B, Brunham RC, Krajden M, Petric M, Skowronski DM, Upton C, Roper RL. The Genome sequence of the SARS-associated coronavirus. Science 300(5624), 1399-404. 2003. Epub 2003 May 01.

Kinsella, E., Martin, S.G., Grolla, A., Czub, M., Feldmann, H., and Flick, R.: Sequence Determination of Crimean-Congo Hemorrhagic Fever Virus L-segment. Virology. 2004, 321(1):23-8.

Czub S, Czub M, Koutsilieri E, Sopper S, Villinger F, Muller JG, Stahl-Hennig C, Riederer P, Ter Meulen V, Gosztonyi G.: Modulation of simian immunodeficiency virus neuropathology by dopaminergic drugs. Acta Neuropathol (Berl). 2004 Mar;107(3):216-26. Epub 2004 Jan 08.

Weingartl, HM, Copps, J, Drebot, MA, Marszal, P, Smith, G, Gren, J, Andonova, M, Pasick, J, Kitching, P, Czub, M: Susceptibility of Pigs and Chickens to SARS Coronavirus. Emerg Infect Dis. 2004 Feb;10(2):179-84.

Stein, V, Czub, M, Hansen, R, Leibold, W, Moore, PF, Vandevelde, M, Zurbriggen, A, Tipold, A.: Characterization of Canine Microglia Cells. Vet Immunol Immunopathol. 2004 May;99(1-2):73-85.

Stein VM, Czub M, Schreiner N, Moore PF, Vandevelde M, Zurbriggen A, Tipold A.: Microglial cell activation in demyelinating canine distemper lesions. J Neuroimmunol. 2004 Aug;153(1-2):122-31.

Moll M, Diederich S, Klenk HD, Czub M, Maisner A.: Ubiquitous activation of the Nipah virus fusion protein does not require a basic amino acid at the cleavage site. J Virol. 2004 Sep;78(18):9705-12.

Weingartl H*, Czub M*, Czub S, Neufeld J, Marszal P, Gren J, Smith G, Jones S, Proulx R, Deschambault Y, Grudeski E, Andonov A, He R, Li Y, Copps J, Grolla A, Dick D, Berry J, Ganske S, Manning L, Cao J.: Immunization with modified vaccinia virus Ankara-based recombinant vaccine against severe acute respiratory syndrome is associated with enhanced hepatitis in ferrets. J Virol. 2004, 78(22):12672-6. *equal contribution

Czub M,  Weingartl H, Czub S, He R, Jingxin C: Evaluation of modified vaccinia virus Ankara based recombinant SARS vaccine in ferrets. Vaccine. 2005 Mar 18;23(17-18):2273-9.

Weingartl H, Czub S, Copps J, Berhane Y, Middleton D,  Marszal P, Gren J, Smith G, Ganske S, Manning L, and Czub M: Invasion of the central nervous system in a porcine host by Nipah virus. J Virol., 2005, 79, 7528-7534.

Carletti F, Di Caro A, Calcaterra S, Grolla A, Czub M, Ippolito G, Capobianchi MR, Horejsh D. Rapid, differential diagnosis of orthopox- and herpesviruses based upon real-time PCR product melting temperature and restriction enzyme analysis of amplicons. J Virol Methods. 2005 Oct;129(1):97-100.

Reviews

Feldmann, H., Czub, M., Jones, S., Dick, D., Garbutt, M., Grolla, A., and Artsob, H.: Emerging and Re-Emerging Infectious Diseases. Med. Microbiol. Immunol. 191, 63-74, 2002.

Flick R, Walpita P, and Czub M: Nipah and Hendra Viral Infections. Tropical Infectious Diseases: Principles, Pathogens, and Practice, 2nd edition, Guerrant RL, Walker DH, Weller PF, eds., Elsevier, in press, 2005.

Laboratory Members:

Shane Jones
Technician
shane_jones@phac-aspc.gc.ca

Roxanne Proulx
Technician
roxanne_proulx@phac-aspc.gc.ca

Bevan Sawatsky
Grad. Student (Ph.D.)
bevan_sawatsky@phac-aspc.gc.ca

Charlene Ranadheera
Grad. Student (Ph.D.)
charlene_ranadheera@phac-aspc.gc.ca

Bonnie Solylo
Grad. Student (Masters)
bonnie_solylo@phac-aspc.gc.ca